By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Medical Imaging. According to news reporting originating from La Jolla, California, by NewsRx correspondents, research stated, "To determine whether intravenous ferumoxytol can be used to effectively label mesenchymal stem cells (MSCs) in vivo and can be used for tracking of stem cell transplants. This study was approved by the institutional animal care and use committee."
Our news editors obtained a quote from the research from BD Biosciences, "Sprague-Dawley rats (6-8 weeks old) were injected with ferumoxytol 48 hours prior to extraction of MSCs from bone marrow. Ferumoxytol uptake by these MSCs was evaluated with fluorescence, confocal, and electron microscopy and compared with results of traditional ex vivo-labeling procedures. The in vivo-labeled cells were subsequently transplanted in osteochondral defects of 14 knees of seven athymic rats and were evaluated with magnetic resonance (MR) imaging up to 4 weeks after transplantation. T2 relaxation times of in vivo-labeled MSC transplants and unlabeled control transplants were compared by using t tests. MR data were correlated with histopathologic results. In vivo-labeled MSCs demonstrated significantly higher ferumoxytol uptake compared with ex vivo-labeled cells. With electron microscopy, iron oxide nanoparticles were localized in secondary lysosomes. In vivo-labeled cells demonstrated significant T2 shortening effects in vitro and in vivo when they were compared with unlabeled control cells (T2 in vivo, 15.4 vs 24.4 msec; P< .05) and could be tracked in osteochondral defects for 4 weeks. Histologic examination confirmed the presence of iron in labeled transplants and defect remodeling. Intravenous ferumoxytol can be used to effectively label MSCs in vivo and can be used for tracking of stem cell transplants with MR imaging."
According to the news editors, the research concluded: "This method eliminates risks of contamination and biologic alteration of MSCs associated with ex vivo-labeling procedures."
For more information on this research see: Iron Administration before Stem Cell Harvest Enables MR Imaging Tracking after Transplantation. Radiology, 2013;269(1):186-197. Radiology can be contacted at: Radiological Soc North America, 820 Jorie Blvd, Oak Brook, IL 60523, USA. (Hindawi Publishing - www.hindawi.com; Radiology - www.hindawi.com/journals/rrp/)
The news editors report that additional information may be obtained by contacting A. Khurana, BD Biosci, Custom Technol Team, La Jolla, CA, United States. Additional authors for this research include F. Chapelin, G. Beck, O.D. Lenkov, J. Donig, H. Nejadnik, S. Messing, N. Derugin, R.C.F. Chan, A. Gaur, B. Sennino, D.M. McDonald, P.J. Kempen, G.A. Tikhomirov, J.H. Rao and H.E. Daldrup-Link (see also Medical Imaging).
Keywords for this news article include: La Jolla, California, United States, Medical Imaging, North and Central America
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