technology and Bioengineering in the Area of DNA Research Reported -->
By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in DNA Research. According to news reporting from Livermore, California, by NewsRx journalists, research stated, "Next-generation sequencing (NGS) is emerging as a powerful tool for elucidating genetic information for a wide range of applications. Unfortunately, the surging popularity of NGS has not yet been accompanied by an improvement in automated techniques for preparing formatted sequencing libraries."
The news correspondents obtained a quote from the research from the Department of Biotechnology and Bioengineering, "To address this challenge, we have developed a prototype microfluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina sequencing. Our system combines droplet-based digital microfluidic (DMF) sample handling with peripheral modules to create a fully-integrated, sample-in library-out platform. In this report, we use our automated system to prepare NGS libraries from samples of human and bacterial genomic DNA. E. coli libraries prepared on-device from 5 ng of total DNA yielded excellent sequence coverage over the entire bacterial genome, with >99% alignment to the reference genome, even genome coverage, and good quality scores. Furthermore, we produced a de novo assembly on a previously unsequenced multi-drug resistant Klebsiella pneumoniae strain BAA-2146 (KpnNDM). The new method described here is fast, robust, scalable, and automated."
According to the news reporters, the research concluded: "Our device for library preparation will assist in the integration of NGS technology into a wide variety of laboratories, including small research laboratories and clinical laboratories."
For more information on this research see: A microfluidic DNA library preparation platform for next-generation sequencing. Plos One, 2013;8(7):e68988. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting H. Kim, Dept. of Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, California, United States. Additional authors for this research include M.J. Jebrail, A. Sinha, Z.W. Bent, O.D. Solberg, K.P. Williams, S.A. Langevin, R.F. Renzi, J.L. Van De Vreugde, R.J. Meagher, J.S. Schoeniger, T.W. Lane, S.S. Branda, M.S. Bartsch and K.D Patel (see also DNA Research).
Keywords for this news article include: Livermore, California, DNA Research, United States, North and Central America.
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