By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators discuss new findings in Biotechnology. According to news reporting originating in Hubei, People's Republic of China, by NewsRx journalists, research stated, "Telomeres are the end structures of chromosomes in mammalian cells; they play a pivotal role in maintaining the stability of the chromosome and become shorter with each cell division. However, several types of tumor cells express telomerase in very high levels to overcome this crisis and achieve the ability to proliferate endlessly."
The news reporters obtained a quote from the research from Cancer Research Institute, "The telomerase inhibitors can partly inhibit tumor cell proliferation and promote apoptosis, but their roles are only limited. Tankyrase is a poly(ADP-ribose) polymerase which has synergistic effect on telomerase, and is expressed in lung cancer cells in high levels. In the present study, antisense oligonucleotides of telomerase (ashTERT) and tankyrase (asTANKS) were used as specific inhibitors to silence the expression of target genes in A549 human lung adenocarcinoma cells by transfection. The results showed that ashTERT and asTANKS suppressed the expression of telomerase and tankyrase significantly; both inhibited the activity of telomerase and the combination group achieved better effect, but only ashTERT shortened the length of telomeres, asTANKS did not. Further studies showed that ashTERT and asTANKS-promoted A549 apoptosis was not mediated by downregulation of the expression of the anti-apoptotic gene BCL-2 or upregulation of the expression of the pro-apoptotic gene BAX, but by adjusting the two isoforms proportion of myeloid cell leukemia-1 (MCL-1) which can interact with tankyrase directly. MCL-1short (MCL-1S), a proapoptotic gene, increased more than MCL-1Long (MCL-1L) which is an anti-apoptotic gene, leading to A549 cell apoptosis and a similar result was obtained in nude mice in vivo."
According to the news reporters, the research concluded: "The present study suggests that combination of the inhibitors of telomerase and tankyrase can be used as a strategy for the treatment of lung cancer in humans."
For more information on this research see: Silencing tankyrase and telomerase promotes A549 human lung adenocarcinoma cell apoptosis and inhibits proliferation. Oncology Reports, 2013;30(4):1745-1752. Oncology Reports can be contacted at: Spandidos Publ Ltd, Pob 18179, Athens, 116 10, Greece (see also technology.html">Biotechnology).
Our news correspondents report that additional information may be obtained by contacting H.D. Lu, Canc Res Inst Wuhan, Wuhan, Hubei, People's Republic of China. Additional authors for this research include Z. Lei, Z.X. Lu, Q.M. Lu, C. Lu, W.Q. Chen, C. Wang, Q. Tang and Q.Z. Kong.
Keywords for this news article include: Asia, Biotechnology, Hubei, Genetics, Oncology, Apoptosis, Tankyrases, Telomerase, Lung Cancer, Adenocarcinomas, Carrier Proteins, Ribonucleoproteins, Cancer Gene Therapy, Phosphotransferases, Glycosyltransferases, Pentosyltransferases, People's Republic of China, DNA Nucleotidyltransferases, RNA-Directed DNA Polymerase
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