By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Proteins. According to news reporting originating in New Cairo, Egypt, by NewsRx journalists, research stated, "A power-free, portable 'Chip EIA' was designed to render the popular Enzyme Linked Immunosorbent Assay (ELISA) more suitable for point-of-care testing. A number of microfluidic platforms have enabled miniaturization of the conventional microtitre plate ELISA, however, they require external pumping systems, valves, and electric power supply."
The news reporters obtained a quote from the research from American University, "The Chip EIA platform has eliminated the need for pumps and valves through utilizing a simple permanent magnet and magnetic nanoparticles. The magnetic nanoparticles act as solid support to capture the target and are then moved through chambers harboring different reagents necessary to perform a sandwich ELISA. The use of magnetic nanoparticles increases the volume-to-surface ratio reducing the assay time to 30 min. Changing the color of horseradish peroxidase (HRP) substrate to green indicates a positive result. In addition, a quantitative read-out was obtained through the use of cellphone camera imaging and analyzing the images using Matlab®. Cell phones, including smart ones, are readily available almost everywhere. The Chip EIA device was used to assay total prostate specific antigen (tPSA) in 19 serum samples. The PSA Chip EIA was tested for accuracy, precision, repeatability, and the results were correlated to the commercial Beckman Colter, Hybritech immunoassay® for determination of tPSA in serum samples with a Pearson correlation coefficient (R(2)=0.96). The lower detection limit of the PSA Chip EIA was 3.2 ng/mL. The assay has 88.9% recovery and good reproducibility (% CV of 6.5)."
According to the news reporters, the research concluded: "We conclude that the developed Chip EIA can be used for detection of protein biomarkers in biological specimens."
For more information on this research see: Power-free chip enzyme immunoassay for detection of prostate specific antigen (PSA) in serum. Biosensors & Bioelectronics, 2013;49():478-84. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news correspondents report that additional information may be obtained by contacting H. Adel Ahmed, Yousef Jameel Science and Technology Research Center (YJ-STRC), The American University in Cairo, AUC Ave, PO Box 74, New Cairo 11835, Egypt (see also Proteins).
Keywords for this news article include: Egypt, Africa, New Cairo, Immunology, Kallikreins, Nanoparticle, Endopeptidases, Nanotechnology, Serine Proteases, Neoplasm Antigens, Peptide Hydrolases, Emerging Technologies, Enzymes and Coenzymes, Biological Tumor Markers, Prostate Specific Antigen, Prostatic Secretory Proteins.
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