By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Researchers detail new data in Biotechnology. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "Induction of murine double minute 2 (MDM2) expression is thought to be a determinant of resistance to p53 gene therapy for cancer. Previous studies have revealed that ribosomal protein L23 (RPL23) inhibits MDM2-mediated p53 degradation through direct binding to MDM2."
Our news journalists obtained a quote from the research from Third Military Medical University, "In addition, ectopically expressed RPL23 was reported to interact with MDM2 in both the nucleus and cytoplasm, by which RPL23 indirectly inhibited MDM2-p53 binding. Based on the known molecular properties of the RPL23 protein, it was speculated that co-transduction of RPL23 may protect wild-type p53 protein from MDM2-mediated inactivation and, thus, improve the effect of delivering therapeutic exogenous p53. To test this hypothesis, we constructed a bicistronic adenoviral vector expressing both the RPL23 and p53 genes (Ad-RPL23/p53) and compared its tumor-suppressor activity in human gastric cancer with that of a single gene vector for p53 (Ad-p53). In the in vivo and in vitro experiments, we observed that treatment with Ad-RPL23/p53 resulted in a stronger antitumor response compared to that obtained using Ad-p53. Moreover, the antitumor response of the bicistronic adenovirus was obtained not only in MGC803 cells (endogenous mutant p53) but also in MKN45 cells (endogenous wild-type p53) which were initially resistant to p53 gene transfer, indicating that co-transduction of RPL23 also expanded the utility of p53 gene therapy. Furthermore, in an orthotopic nude mouse model of human gastric cancer, we found that the survival benefit was greater after Ad-RPL23/p53 treatment than after Ad-p53."
According to the news editors, the research concluded: "Taken together, the data presented here demonstrate that co-transduction of RPL23 enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in models of human gastric cancer and support the use of this strategy for cancer treatment."
For more information on this research see: Co-transduction of ribosomal protein L23 enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in human gastric cancer. Oncology Reports, 2013;30(4):1989-1995. Oncology Reports can be contacted at: Spandidos Publ Ltd, Pob 18179, Athens, 116 10, Greece (see also technology.html">Biotechnology).
Our news journalists report that additional information may be obtained by contacting Y.F. Zhang, Third Military Medical University, Southwest Hosp, Dept. of Gastroenterol, Chongqing 400038, People's Republic of China. Additional authors for this research include B.C. Zhang, A.R. Zhang, T.T. Wu, J. Liu, L.F. Yu, W.X. Wang, J.F. Gao, D.C. Fang and Z.G. Rao.
Keywords for this news article include: Asia, Biotechnology, Genetics, Oncology, Chongqing, Treatment, Therapeutics, Bioengineering, Gastric Cancer, Gastroenterology, Ribosomal Proteins, Cancer Gene Therapy, People's Republic of China
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