By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on DNA Research is now available. According to news reporting from Shandong, People's Republic of China, by NewsRx journalists, research stated, "In this work, we fabricated a novel electrochemical immunosensor for detection of DNA methylation, analysis of DNA MTase activity and screening of MTase inhibitor. The immunosensor was on the basis of methyl binding domain protein of MeCP2 as DNA CpG methylation recognization unit, anti-His tag antibody as 'immuno-bridge' and horseradish peroxidase labeled immuneglobulin G functionalized gold nanoparticles (AuNPs-IgG-HRP) as signal amplification unit."
The news correspondents obtained a quote from the research from Shandong Agricultural University, "In the presence of M. SssI MTase, the symmetrical sequence of 5'-CCGG-3' was methylated and then recognized by MeCP2 protein. By the immunoreactions, anti-His tag antibody and AuNPs-IgG-HRP was captured on the electrode surface successively. Under the catalysis effect of HRP towards hydroquinone oxidized by H2O2, the electrochemical reduction signal of benzoquinone was used to analyze M. SssI MTase activity. The electrochemical reduction signal demonstrated a wide linear relationship with M. SssI concentration ranging from 0.05 unit/mL to 90 unit/mL, achieving a detection limit of 0.017 unit/mL (S/N=3). The most important advantages of this method were its high sensitivity and good selectivity, which enabled the detection of even one-base mismatched sequence."
According to the news reporters, the research concluded: "In addition, we also verified that the developed method could be applied for screening the inhibitors of DNA MTase and for developing new anticancer drugs."
For more information on this research see: Ultrasensitive electrochemical immunoassay for DNA methyltransferase activity and inhibitor screening based on methyl binding domain protein of MeCP2 and enzymatic signal amplification. Biosensors & Bioelectronics, 2013;49():39-45. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting H. Yin, College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Taiwan. Additional authors for this research include Y. Zhou, Z. Xu, M. Wang and S. Ai (see also DNA Research).
Keywords for this news article include: Asia, Antibodies, Shandong, Chemistry, Immunology, DNA Research, Blood Proteins, Electrochemical, Immunoglobulins, Methyltransferases, Enzymes and Coenzymes, People's Republic of China.
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