By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Life Science Research have been published. According to news reporting originating from Norfolk, Virginia, by NewsRx correspondents, research stated, "High-amplitude electric pulses of nanosecond duration, also known as nanosecond pulsed electric field (nsPEF), are a novel modality with promising applications for cell stimulation and tissue ablation. However, key mechanisms responsible for the cytotoxicity of nsPEF have not been established."
Our news editors obtained a quote from the research from Old Dominion University, "We show that the principal cause of cell death induced by 60-or 300-ns pulses in U937 cells is the loss of the plasma membrane integrity ('nanoelectroporation'), leading to water uptake, cell swelling, and eventual membrane rupture. Most of this early necrotic death occurs within 1-2 hr after nsPEF exposure. The uptake of water is driven by the presence of pore-impermeable solutes inside the cell, and can be counterbalanced by the presence of a pore-impermeable solute such as sucrose in the medium. Sucrose blocks swelling and prevents the early necrotic death; however the long-term cell survival (24 and 48 hr) does not significantly change. Cells protected with sucrose demonstrate higher incidence of the delayed death (6-24 hr post nsPEF). These cells are more often positive for the uptake of an early apoptotic marker dye YO-PRO-1 while remaining impermeable to propidium iodide. Instead of swelling, these cells often develop apoptotic fragmentation of the cytoplasm. Caspase 3/7 activity increases already in 1 hr after nsPEF and poly-ADP ribose polymerase (PARP) cleavage is detected in 2 hr. Staurosporin-treated positive control cells develop these apoptotic signs only in 3 and 4 hr, respectively. We conclude that nsPEF exposure triggers both necrotic and apoptotic pathways. The early necrotic death prevails under standard cell culture conditions, but cells rescued from the necrosis nonetheless die later on by apoptosis."
According to the news editors, the research concluded: "The balance between the two modes of cell death can be controlled by enabling or blocking cell swelling."
For more information on this research see: Two modes of cell death caused by exposure to nanosecond pulsed electric field. Plos One, 2013;8(7):e70278. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news editors report that additional information may be obtained by contacting O.N. Pakhomova, Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, United States. Additional authors for this research include B.W. Gregory, I. Semenov and A.G Pakhomov (see also Life Science Research).
Keywords for this news article include: Norfolk, Virginia, United States, Life Science Research, North and Central America.
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