By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Proteins have been presented. According to news reporting out of Shanghai, People's Republic of China, by NewsRx editors, research stated, "We developed a novel polyacrylamide gel electrophoresis (PAGE) method to stack and separate human hemoglobins (Hbs) based on the concept of moving reaction boundary (MRB). This differs from the classic isotachophoresis (ITP)-based stacking PAGE in the aspect of buffer composition, including the electrode buffer (pH 8.62 Tris-Gly), sample buffer (pH 6.78 Tris-Gly), and separation buffer (pH 8.52 Tris-Gly)."
Our news journalists obtained a quote from the research from Fudan University, "In the MRB-PAGE system, a transient MRB was formed between alkaline electrode buffer and acidic sample buffer, being designed to move toward the anode. Hbs carried partial positive charges in the sample buffer due to its pH below pI values of Hbs, resulting in electromigrating to the cathode. Hbs would carry negative charges quickly when migrated into the alkaline electrode buffer and be transported to the anode until meeting the sample buffer again. Thus, Hbs were stacked within a MRB until the transient MRB reached the separation buffer and then separated by zone electrophoresis with molecular sieve effect of the gel. The experimental results demonstrated that there were three clear and sharp protein zones of Hbs (HbA(1c), HbA(0), and HbA(2)) in MRB-PAGE, in contrast to only one protein zone (HbA(0)) in ITP-PAGE for large-volume loading (a parts per thousand yen15 mu l), indicating high stacking efficiency, separation resolution, and good sensitivity of MRB-PAGE. In addition, MRB-PAGE was performed in a conventional slab PAGE device, requiring no special device."
According to the news editors, the research concluded: "Thus, it could be widely used in separation and analysis of diluted protein in a standard laboratory."
For more information on this research see: Novel moving reaction boundary-induced stacking and separation of human hemoglobins in slab polyacrylamide gel electrophoresis. Analytical and Bioanalytical Chemistry, 2013;405(26):8587-8595. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
Our news journalists report that additional information may be obtained by contacting Y.Y. Tang, Fudan University, Dept. of Chem, Shanghai 200433, People's Republic of China. Additional authors for this research include H.Y. Wang, L. Chen, S. Li, C.G. Guo, H.Z. Fan, C.X. Cao and L.Y. Fan (see also Proteins).
Keywords for this news article include: Asia, Shanghai, Hemoglobins, Blood Proteins, People's Republic of China
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