By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators discuss new findings in Biotechnology. According to news originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "Nanoparticles (NPs) with modification of brain-targeting molecules have been extensively exploited for therapeutic gene delivery across the blood brain barrier (BBB). As one of the effective RNA interference (RNAi) approaches, short hairpin RNA (shRNA) has been proved to be promising in the field of gene therapy."
Our news journalists obtained a quote from the research from Fudan University, "Apoptosis signal-regulating kinase 1 (Ask1) has been reported to be an important target for gene therapy against cerebral ischemia reperfusion injury. In this study, dendrigraft poly-L-lysine (DGL) was decorated by dermorphin (a mu-opiate receptor agonist) through PEG for efficient brain-targeting, then complexed with anti-Ask1 shRNA plasmid DNA, yielding the DGL-PEG-dermorphin/shRNA NPs. The DGL-PEG-dermorphin/shRNA NPs were characterized and estimated the brain-targeting ability. In vitro, increased cellular uptake and transfection efficiency were explored; in vivo, preferable accumulation and gene transfection in brain were showed in images. The DGL-PEG-dermorphin/shRNA NPs also revealed high efficiency of neuroprotection. As a result of RNAi, corresponding mRNA was distinctly degraded, expression of Ask1 protein was obviously suppressed, apoptotic cell death was apparently decreased and cerebral infarct area was significantly reduced."
According to the news editors, the research concluded: "Above all, DGL-PEG-dermorphin/shRNA NPs were proved to be efficient and safe for brain-targeting RNAi neuroprotection against cerebral ischemia reperfusion injury."
For more information on this research see: Brain-targeting delivery for RNAi neuroprotection against cerebral ischemia reperfusion injury. Biomaterials, 2013;34(35):8949-8959. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news correspondents report that additional information may be obtained from S. An, Fudan University, Sch Pharm, Dept. of Pharmaceut, Key Lab Smart Drug DeliveryMinist Educ, Shanghai 201203, People's Republic of China. Additional authors for this research include Y.Y. Kuang, T. Shen, J.F. Li, H.J. Ma, Y.B. Guo, X. He and C. Jiang (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Shanghai, Gene Therapy, Bioengineering, Brain Ischemia, Medical Devices, Blood Transfusion, Transfusion Medicine, Cardiovascular Diseases, People's Republic of China, Ischemia-Reperfusion Injury
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