By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Life Science Research is the subject of a report. According to news reporting originating from Bagnols sur Ceze, France, by NewsRx correspondents, research stated, "The continuing development of nanotechnology necessitates the reliable assessment of potential adverse health consequences associated with human exposures. The physicochemical properties of nanomaterials can be responsible for unexpected interactions with components of classical toxicity assays, which may generate erroneous interpretations."
Our news editors obtained a quote from the research from Alternative Energies and Atomic Energy Commission, "In this paper, we describe how particle interference can be observed in in vitro toxicity tests (CellTiter Blue, CyQUANT, WST-1 and CellTiter-Glo assay) and in cell biology tests using flow cytometry (cell cycle analysis). We used cobalt oxide (Co3O4) particles as an example, but these assays can be performed, in principle, regardless of the nanoparticle considered. We have shown that cobalt particles interfere with most of these tests. We adapted the protocol of the CellTiter-Glo assay to circumvent this interference and demonstrated that, using this protocol, the toxicity level is consistent with results obtained using the clonogenic assay, which is considered to be the reference test. Before assessing particle toxicity using in vitro toxicity tests, interference testing should be performed to avoid false interpretations."
According to the news editors, the research concluded: "Furthermore, in some cases of interference, protocol adaptation can be considered to allow the reliable use of these quick and convenient in vitro tests."
For more information on this research see: In vitro assessment of cobalt oxide particle toxicity: Identifying and circumventing interference. Toxicology in Vitro, 2013;27(6):1699-1710. Toxicology in Vitro can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Toxicology in Vitro - www.elsevier.com/wps/product/cws_home/800)
The news editors report that additional information may be obtained by contacting C. Darolles, CEA, DSV, IBEB, Lab Biochim Syst Perturb, F-30207 Bagnols Sur Ceze, France. Additional authors for this research include N. Sage, J. Armengaud and V. Malard (see also Life Science Research).
Keywords for this news article include: France, Europe, Cobalt, Bagnols sur Ceze, Transition Elements, Life Science Research
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