By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- New research on Biotechnology is the subject of a report. According to news reporting from Suzhou, People's Republic of China, by NewsRx journalists, research stated, "Angiopoietin-2 (Ang2) has been shown highly expressed in resected human pancreatic carcinoma samples, and has tightly combination with tumor angiogenesis, but the role in metastasis of it is less clear. We were, therefore, interested in exploring the effects of Ang2 silencing on the invasion and metastasis of pancreatic carcinoma."
The news correspondents obtained a quote from the research from First Affiliated Hospital, "Lentivirus (LV)-mediated Ang2 small hairpin RNA (LV-RNAi) and mock lentivirus (LV-NC) were transfected into pancreatic carcinoma cell line MIA PaCa-2. Groups were designed in this study: the control group (MIA PaCa-2 cells), the LV-NC group (cells transfected with the LV-NC), the LV-RNAi-KD1 group (cells transfected with LV-RNAi of knock down sequence (1) and the LV-RNAi-KD2 group (cells transfected with LV-RNAi of knock down sequence (2). Boyden chamber transwell assay was used to detect the cell invasion change. The protein levels of Ang2, MMP-2, and MMP-9 gene and mRNA level of MMP-2, MMP-9 were detected by Western blot and real-time polymerase chain reaction, respectively. Orthotopic pancreatic carcinoma xenotransplantation model were successfully built with MIA PaCa-2 cells injection. After treatment with intraperitoneal injection of LV-RNAi-KD2 (LV-RNAi), mice growth, liver function test, tumor volume and peritoneal metastatic numbers were observed and counted. Moreover, expression of Ang2, MMP-2, MMP-9 were measured by immunohistochemistry. Ang2 expression were successfully knocked down in two LV-RNAi groups, especially in the LV-RNAi-KD2group. Compared with the control group and the LV-NC group, the mRNA and protein level of MMP-2 gene were downregulated significantly in LV-RNAi groups, also the invasion cell number decreased in boyden chamber transwell assay after LV-RNAi transfection. Meanwhile, no obvious MMP-9 gene expression changes were found among all the groups. LV-RNAi injection inhibited pancreatic carcinoma metastasis and growth in vivo by downregulating the expression of MMP-2 not MMP-9. Most importantly, LV-mediated gene therapy with Ang2 knockdown exhibited almost no toxicity in vivo."
According to the news reporters, the research concluded: "These findings demonstrate that Ang2 gene silencing exert an anti-metastasis effect in vitro and in vivo, and Ang2 targeted gene therapy has the potential to serve as a novel way for pancreatic carcinoma treatment."
For more information on this research see: Knockdown of angiopoietin-2 suppresses metastasis in human pancreatic carcinoma by reduced matrix metalloproteinase-2. Molecular Biotechnology, 2013;53(3):336-44. Molecular Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Molecular Biotechnology - www.springerlink.com/content/1073-6085/)
Our news journalists report that additional information may be obtained by contacting Z.X. Zhang, Dept. of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, People's Taiwan. Additional authors for this research include J. Zhou, Y. Zhang, D.M. Zhu, D.C. Li and H. Zhao (see also technology.html">Biotechnology).
Publisher contact information for the journal Molecular Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Asia, Biotechnology, Suzhou, Oncology, Carcinoma, Treatment, Proteomics, Gelatinases, Collagenases, Angiopoietins, Bioengineering, Gastroenterology, Metalloproteases, Pancreatic Cancer, Peptide Hydrolases, Angiogenic Proteins, Cancer Gene Therapy, Enzymes and Coenzymes, Metalloendopeptidases, People's Republic of China.
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