By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Immunology. According to news reporting originating in Turku, Finland, by NewsRx journalists, research stated, "A comprehensive in vitro assessment of two commercial metal oxide nanoparticles, TiO2 and ZnO, was performed using human monocyte-derived macrophages (HMDM), monocyte-derived dendritic cells (MDDC), and Jurkat T cell leukemia-derived cell line. TiO2 nanoparticles were found to be non-toxic whereas ZnO nanoparticles caused dose-dependent cell death."
The news reporters obtained a quote from the research from the University of Turku, "Subsequently, global gene expression profiling was performed to identify transcriptional response underlying the cytotoxicity caused by ZnO nanoparticles. Analysis was done with doses 1 g/ml and 10 g/ml after 6 and 24 h of exposure. Interestingly, 2703 genes were significantly differentially expressed in HMDM upon exposure to 10 g/ml ZnO nanoparticles, while in MDDCs only 12 genes were affected. In Jurkat cells, 980 genes were differentially expressed. It is noteworthy that only the gene expression of metallothioneins was upregulated in all the three cell types and a notable proportion of the genes were regulated in a cell type-specific manner. Gene ontology analysis revealed that the top biological processes disturbed in HMDM and Jurkat cells were regulating cell death and growth. In addition, genes controlling immune system development were affected. Using a panel of modified ZnO nanoparticles, we obtained an additional support that the cellular response to ZnO nanoparticles is largely dependent on particle dissolution and show that the ligand used to modify ZnO nanoparticles modulates Zn(2+) leaching."
According to the news reporters, the research concluded: "Overall, the study provides an extensive resource of transcriptional markers for mediating ZnO nanoparticle-induced toxicity for further mechanistic studies, and demonstrates the value of assessing nanoparticle responses through a combined transcriptomics and bioinformatics approach."
For more information on this research see: Gene expression profiling of immune-competent human cells exposed to engineered zinc oxide or titanium dioxide nanoparticles. Plos One, 2013;8(7):e68415. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news correspondents report that additional information may be obtained by contacting S. Tuomela, Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Turku, Finland. Additional authors for this research include R. Autio, T. Buerki-Thurnherr, O. Arslan, A. Kunzmann, B. Andersson-Willman, P. Wick, S. Mathur, A. Scheynius, H.F. Krug, B. Fadeel and R. Lahesmaa (see also Immunology).
Keywords for this news article include: Turku, Europe, Finland, Chemicals, Chemistry, Monocytes, Immunology, Zinc Oxide, Blood Cells, Engineering, Nanoparticle, Nanotechnology, Zinc Compounds, Titanium Dioxide, Bone Marrow Cells, Emerging Technologies, Mononuclear Leukocytes, Hemic and Immune Systems, Mononuclear Phagocyte System.
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