By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Enzymes and Coenzymes is now available. According to news reporting out of Jena, Germany, by NewsRx editors, research stated, "In this article, we show that the protein disulfide isomerase 2 of Chlamydomonas reinhardtii is involved in circadian rhythm regulation and interacts with a peroxiredoxin specifically during night phase. Furthermore, the recombinant protein could be characterized as a redox-active enzyme.Protein disulfide isomerases (PDIs) are known to play important roles in the folding of nascent proteins and in the formation of disulfide bonds."
Our news journalists obtained a quote from the research from the University of Jena, "Recently, we identified a PDI from Chlamydomonas reinhardtii (CrPDI2) by a mass spectrometry approach that is specifically enriched by heparin affinity chromatography in samples taken during the night phase. Here, we show that the recombinant CrPDI2 is a redox-active protein. It is reduced by thioredoxin reductase and catalyzes itself the reduction of insulin chains and the oxidative refolding of scrambled RNase A. By immunoblots, we confirm a high-amplitude change in abundance of the heparin-bound CrPDI2 during subjective night. Interestingly, we find that CrPDI2 is present in protein complexes of different sizes at both day and night. Among three identified interaction partners, one (a 2-cys peroxiredoxin) is present only during the night phase. To study a potential function of CrPDI2 within the circadian system, we have overexpressed its gene. Two transgenic lines were used to measure the rhythm of phototaxis. In the transgenic strains, a change in the acrophase was observed."
According to the news editors, the research concluded: "This indicates that CrPDI2 is involved in the circadian signaling pathway and, together with the night phase-specific interaction of CrPDI2 and a peroxiredoxin, these findings suggest a close coupling of redox processes and the circadian clock in C. reinhardtii."
For more information on this research see: Protein Disulfide Isomerase 2 of Chlamydomonas reinhardtii Is Involved in Circadian Rhythm Regulation. Molecular Plant, 2013;6(5):1503-1517. Molecular Plant can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Molecular Plant - mplant.oxfordjournals.org)
Our news journalists report that additional information may be obtained by contacting A. Filonova, University of Jena, Inst Gen Bot & Plant Physiol, D-07743 Jena, Germany. Additional authors for this research include P. Haemsch, C. Gebauer, W. Weisheit and V. Wagner (see also Enzymes and Coenzymes).
Keywords for this news article include: Jena, Ions, Europe, Germany, Peroxidases, Electrolytes, Peroxiredoxins, Inorganic Chemicals, Enzymes and Coenzymes, Protein Disulfide-Isomerases, Sulfur-Sulfur Bond Isomerases
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