By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Life Science Research have been published. According to news reporting originating in Murcia, Spain, by NewsRx journalists, research stated, "Oviduct fluid increases the time required for digestion of the zona pellucida (ZP) by proteolytic enzymes (ZP hardening). This effect has been associated with levels of monospermy after in vitro fertilization (IVF) in the pig and cow, but the possible existence of a directly proportional relationship between hardening and monospermy remains unknown."
The news reporters obtained a quote from the research from Institute for Biomedical Research, "To investigate whether variations in hardening of different oviductal fluids (OFs) are correlated with variations in levels of monospermy after IVF, porcine oocytes were incubated with three batches of OFs known to produce different ZP hardening effects (3, 7, and 25 min); after IVF, monospermy levels were 0%, 14.58% +/- 5.14%, and 35.14% +/- 7.95%, respectively. These results could partially explain the lack of polyspermy found during in vivo fertilization in pigs (with a hardened oviductal ZP) compared with levels found during IVF (with no hardened ZP). Using the bovine model, OF was fractionated by heparin affinity chromatography, and the hardening effect on the ZP was tested for each fraction obtained from a linear gradient of sodium chloride concentration. The highest effect was obtained with the fraction eluted with 0.4 M sodium chloride. Fractions with high-level or low-level effects were processed by on-chip electrophoresis and high-performance liquid chromatography-tandem mass spectrometry."
According to the news reporters, the research concluded: "A list of potential proteins responsible for this effect includes OVGP1 and members of the HSP and PDI families."
For more information on this research see: Identification of Potential Oviductal Factors Responsible for Zona Pellucida Hardening and Monospermy During Fertilization in Mammals. Biology of Reproduction, 2013;89(3):179-186. Biology of Reproduction can be contacted at: Soc Study Reproduction, 1691 Monroe St,Suite # 3, Madison, WI 53711-2021, USA (see also Life Science Research).
Our news correspondents report that additional information may be obtained by contacting I. Mondejar, Inst Biomed Res Murcia, Murcia, Spain. Additional authors for this research include I. Martinez-Martinez, M. Aviles and P. Coy.
Keywords for this news article include: Spain, Murcia, Europe, Chemicals, Chemistry, Sodium Chloride, Life Science Research
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