By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biological Factors have been published. According to news reporting originating from Reggio di Calabria, Italy, by NewsRx correspondents, research stated, "A simple, rapid, and sensitive method based on simultaneous protein precipitation and extraction of aflatoxin M-1 (AFM(1)) followed by dispersive liquid-liquid microextraction (DLLME) and ultrahigh pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) analysis was developed for the determination of AFM(1) in milk samples. In order to precipitate the proteins and extract AFM(1) from milk, a sample pretreatment using acetonitrile and NaCl as the extraction/denaturant solvent and salting-out agent, respectively, was optimised."
Our news editors obtained a quote from the research from the University of the Mediterranean, "Subsequently, the acetonitrile (upper) phase, containing AFM(1), was used as the disperser solvent in DLLME, and extractant (chloroform) and water were added in turn to the extract to perform the DLLME process. The main parameters affecting the extraction efficiency of the whole analytical procedure, such as acetonitrile volume, amount of salt, type and volume of extractant and water volume, were carefully optimised by experimental design. Under optimum conditions, the developed method provides an enrichment factor of 33 and detection and quantification limits (0.6 and 2.0 ng kg(-1), respectively) below the maximum levels imposed by current regulations for AFM(1) in milk and infant milk formulae. Recoveries (61.3-75.3 %) and repeatability (RSD < 10, n = 3), tested in different types of milk at four AFM(1) levels, met the performance criteria required by EC Regulation No. 401/2006. Moreover, the matrix effect on the signal intensity of the analyte was negligible."
According to the news editors, the research concluded: "The proposed method provides a rapid extraction and an accurate determination of AFM(1) in milk and formula milk using a simple and inexpensive sample preparation procedure."
For more information on this research see: Rapid analysis of aflatoxin M-1 in milk using dispersive liquid-liquid microextraction coupled with ultrahigh pressure liquid chromatography tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 2013;405(26):8645-8652. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
The news editors report that additional information may be obtained by contacting L. Campone, Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89124 Reggio Di Calabria, Italy. Additional authors for this research include A.L. Piccinelli, R. Celano, M. Russo and L. Rastrelli (see also Biological Factors).
Keywords for this news article include: Italy, Europe, Aflatoxins, Mycotoxins, Acetonitriles, Reggio di Calabria, Biological Factors
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