By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on DNA Research. According to news originating from Xi'an, People's Republic of China, by NewsRx correspondents, research stated, "A signal-on impedimetric electrochemical DNA (E-DNA) sensor using gold nanoparticles (AuNPs) as tag was developed for highly sensitive detection of DNA hybridization. A probe ssDNA (PDNA) was immobilized by forming an amide between the -NH2 moiety at the 5'-terminus of PDNA and the -COOH group at self-assembled 11-mercaptoundecanoic acid on a gold electrode."
Our news journalists obtained a quote from the research from Shaanxi Normal University, "Subsequently, AuNPs were attached to the -SH moiety at the 3'-terminus of the immobilized PDNA by S-Au interaction, and then functionalized with -OH by immersing the electrode in dithiothreitol solution. In the absence of the target DNA, the flexible single-stranded PDNA supports efficient contact between AuNP tag and electrode, ensuring a low electron transfer resistance (Ret) of the E-DNA sensor using the [Fe(CN)(6)](3-/4-) redox probe. Upon hybridization, a rigid probe-target duplex is formed, which pushes the AuNP tag away from the electrode and increases the distance between AuNP tag and the electrode, thereby increasing the Ret of the E-DNA sensor. Based on hybridization-induced conformational changes, the E-DNA sensor shows an increased Ret response when the target DNA concentration is increased from 5 fM to 500 pM."
According to the news editors, the research concluded: "Furthermore, the E-DNA sensor showed differentiation abilities for single-base mismatch."
For more information on this research see: Signal-on impedimetric electrochemical DNA sensor using dithiothreitol modified gold nanoparticle tag for highly sensitive DNA detection. Analytica Chimica Acta, 2013;799():36-43. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news correspondents report that additional information may be obtained from C.P. Wang, Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Appl Surface & Colloid Chem, Minist Educ, Xian 710062, People's Republic of China. Additional authors for this research include X.Q. Yuan, X.H. Liu, Q. Gao, H.L. Qi and C.X. Zhang (see also DNA Research).
Keywords for this news article include: Asia, Xi'an, Chemistry, DNA Research, Dithiothreitol, Nanotechnology, Sugar Alcohols, Thioglycosides, Electrochemical, Gold Nanoparticles, Emerging Technologies, People's Republic of China
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