By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Researchers detail new data in Epithelial Cells. According to news originating from Kyotanabe, Japan, by NewsRx correspondents, research stated, "Gelatinous drop-like corneal dystrophy (GDLD) is characterized by subepithelial amyloid deposition that engenders severe vision loss. The exact mechanism of this disease has yet to be elucidated."
Our news journalists obtained a quote from the research from Doshisha University, "No fundamental treatment exists. This study was conducted to establish an immortalized corneal epithelial cell line to be used as a GDLD disease model. A corneal tissue specimen was obtained from a GDLD patient during surgery. Corneal epithelial cells were enzymatically separated from the cornea and were dissociated further into single cells. The epithelial cells were immortalized by the lentiviral transduction of the simian virus 40 (SV40) large T antigen and human telomerase reverse transcriptase (hTERT) genes. For the immortalized cells, proliferative kinetics, gene expressions, and functional analyses were performed. The immortalized corneal epithelial cells continued to proliferate despite cumulative population doubling that exceeded 100. The cells showed almost no sign of senescence and displayed strong colony-forming activity. The cells exhibited a low epithelial barrier function as well as decreased expression of tight-junction-related proteins claudin 1 and 7. Using the immortalized corneal epithelial cells derived from a GDLD patient, we tested the possibility of gene therapy. We established an immortalized corneal epithelial cell line from a GDLD patient. The immortalized cells exhibited cellular phenotypes similar to those of in vivo GDLD."
According to the news editors, the research concluded: "The immortalized cells are thought to be useful for the development of new therapies for treating GDLD corneas and for elucidation of the pathophysiology of GDLD."
For more information on this research see: Establishment of a Human Corneal Epithelial Cell Line Lacking the Functional TACSTD2 Gene as an In Vitro Model for Gelatinous Drop-Like Dystrophy. Investigative Ophthalmology & Visual Science, 2013;54(8):5701-5711. Investigative Ophthalmology & Visual Science can be contacted at: Assoc Research Vision Ophthalmology Inc, 12300 Twinbrook Parkway, Rockville, MD 20852-1606, USA. (The Association for Research in Vision and Ophthalmology - www.arvo.org; Investigative Ophthalmology & Visual Science - www.iovs.org/)
The news correspondents report that additional information may be obtained from K. Kitazawa, Doshisha University, Fac Life & Med Sci, Dept. of Biomed Engn, Kyotanabe, Japan. Additional authors for this research include S. Kawasaki, K. Shinomiya, K. Aoi, A. Matsuda, T. Funaki, K. Yamasaki, M. Nakatsukasa, N. Ebihara, A. Murakami, J. Hamuro and S. Kinoshita (see also Epithelial Cells).
Keywords for this news article include: Asia, Biotechnology, Japan, Kyotanabe, Gene Therapy, Bioengineering, Epithelial Cells
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