By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Research findings on Biotechnology are discussed in a new report. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "Non-viral vector transfection efficiency is an issue affecting the clinical application of stem cell gene therapy. This study makes use of the synergistic effect of combining ultrasound (US) with microbubbles (MB) and polyethylenimine (PEI) to increase DNA transfection efficiency, which will enhance the efficiency of gene transfer to bone marrow stromal cells (BMSCs)."
Our news journalists obtained a quote from the research from Third Military Medical University, "The optimal parameters for primary-cultured rat-BMSC DNA transfection were examined. The study was arranged based on uniform design. Using a construct containing hepatocyte growth factor (HGF) tagged with enhanced green fluorescent protein (pEGFP-HGF) as example, the mixture of BMSCs, MB, and PEI:DNA complex were exposed to US with frequency of 1 MHz and 10% duty cycle pulses. Other factors such as acoustic intensity (Q), MB dosage, and total treatment time (T) were also tested. The results were analyzed by regression analysis. Using the best match of parameters, Q=0.6 W/cm(2), MB=106/ml, T=30 s, different groups were compared. The cooperativity of MB-mediated US and PEI enhanced the gene transfection efficiency by nearly 38-times compared to the DNA without US group. Furthermore, the expression of HGF protein was confirmed by Western blot. The eGFP could be not only seen mainly at the cytoplasm, but also seen in the nucleus in a small proportion of the cells (
According to the news editors, the research concluded: "Our results provide useful information in establishing a novel non-viral transfection method, which may be applied to clinical application in stem cell gene therapy."
For more information on this research see: DNA transfection of bone marrow stromal cells using microbubble-mediated ultrasound and polyethylenimine: an in vitro study. Cell Biochemistry and Biophysics, 2013;66(3):775-86. Cell Biochemistry and Biophysics can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Cell Biochemistry and Biophysics - www.springerlink.com/content/1085-9195/)
Our news journalists report that additional information may be obtained by contacting P. Li, Dept. of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, People's Taiwan. Additional authors for this research include Y. Gao, Z. Liu, K. Tan, Z. Zuo, H. Xia, D. Yang, Y. Zhang and D. Lu (see also technology.html">Biotechnology).
Publisher contact information for the journal Cell Biochemistry and Biophysics is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Asia, Biotechnology, Chongqing, Ultrasound, Bone Marrow, DNA Research, Gene Therapy, Bone Research, Immune System, Stromal Cells, Bioengineering, Connective Tissue Cells, Hepatocyte Growth Factor, People's Republic of China.
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