By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Tetracyclines. According to news reporting from Fuzhou, People's Republic of China, by NewsRx journalists, research stated, "A new signal amplification strategy for sensitive electrochemical determination of tetracycline (TC) was developed by using platinum-catalyzed hydrogen evolution reaction (HER) on an anti-TC antibody-modified immunosensor. To construct such a HER, platinum nanoparticles were initially deposited to graphene nanosheets, and the as-synthesized platinum/graphene nanosheets (PtGN) were then used for the labeling of tetracycline-bovine serum albumin conjugates (TC-BSA)."
The news correspondents obtained a quote from the research from Fuzhou University, "With a competitive immunoassay format, the resulting immunosensor was immersed into a platinum developer solution containing 1.0 mM PtCl42-, 0.1 M formate (reductant) and 0.5% Tween 80 (pH 6.5) to promote the platinum growth. The amplified electrochemical signal mainly derived from the platinum-catalyzed HER in an acidic medium containing 10 mM HCl and 1.0 M KCl. Two labeling methods and assay protocols including Pt-labeled TC-BSA and PtGN-labeled TC-BSA with or without the platinum enhancement were investigated for determination of target TC, respectively, and improved analytical features were obtained with graphene nanosheets and platinum growth mechanism. With PtGN-labeled TC-BSA, the effects of incubation time for antigen-antibody reaction and deposition time of platinum on the currents of the immunosensors were also studied. The strong attachment of TC-BSA to the PtGN resulted in a good repeatability and intermediate precision down to 9.8%. The dynamic concentration range spanned from 0.05 ng/mL to 100 ng/mL tetracycline with a low detection limit of 6 pg/mL at the 3s(blank) level."
According to the news reporters, the research concluded: "In addition, the methodology was further validated with tetracycline spiked samples including honey, milk and peanut, and the recoveries were 86-118%."
For more information on this research see: Platinum-catalyzed hydrogen evolution reaction for sensitive electrochemical immunoassay of tetracycline residues. Journal of Electroanalytical Chemistry, 2013;704():111-117. Journal of Electroanalytical Chemistry can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Tetracyclines).
Our news journalists report that additional information may be obtained by contacting X.H. Que, Fuzhou Univ, Dept. of Chem & Chem Engn, Minist Educ & Fujian Prov, Key Lab Anal & Detect Food Safety, Fuzhou 350108, People's Republic of China. Additional authors for this research include X. Chen, L.B. Fu, W.Q. Lai, J.Y. Zhuang, G.N. Chen and D.P. Tang.
Keywords for this news article include: Asia, Antibodies, Gases, Fuzhou, Elements, Hydrogen, Chemistry, Immunology, Nanosheets, Hydrocarbons, Naphthacenes, Tetracyclines, Blood Proteins, Nanotechnology, Electrochemical, Immunoglobulins, Inorganic Chemicals, Emerging Technologies, People's Republic of China
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