By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on DNA Research have been presented. According to news reporting originating from Montreal, Canada, by NewsRx correspondents, research stated, "DNA intercalation has found many diagnostic and therapeutic applications. Here, we propose the use of simple DNA intercalators, such as ethidium bromide, as tools to facilitate the error-free self-assembly of DNA nanostructures."
Our news editors obtained a quote from the research from McGill University, "We show that ethidium bromide can influence DNA self-assembly, decrease the formation of oligomeric side products, and cause libraries of multiple equilibrating structures to converge into a single product. Using a variety of 2D- and 3D-DNA systems, we demonstrate that intercalators present a powerful alternative for the adjustment of strand-end alignment, favor the formation of fully duplexed 'closed' structures, and create an environment where the smallest, most stable structure is formed. A new 3D-DNA motif, the ninja star, was self-assembled in quantitative yield with this method."
According to the news editors, the research concluded: "Moreover, ethidium bromide can be readily removed using isoamyl alcohol extractions combined with intercalator-specific spin columns, thereby yielding the desired ready-to-use DNA structure."
For more information on this research see: Intercalators as Molecular Chaperones in DNA Self-Assembly. Journal of the American Chemical Society, 2013;135(30):11283-11288. Journal of the American Chemical Society can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)
The news editors report that additional information may be obtained by contacting A.A. Greschner, McGill University, Center Self Assembled Chem Struct, Montreal, PQ H3A 0B8, Canada. Additional authors for this research include K.E. Bujold and H.F. Sleiman (see also DNA Research).
Keywords for this news article include: Quebec, Canada, Montreal, DNA Research, North and Central America, Molecular Chaperones
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