By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Nanomedicine have been presented. According to news reporting out of Guangdong, People's Republic of China, by NewsRx editors, research stated, "A local nanotherapy (LNT) combining the therapeutic efficacy of trans-arterial embolization, nanoparticles, and p53 gene therapy has been previously presented. The study presented here aimed to further improve the incomplete tumor eradication and limited survival enhancement and to elucidate the molecular mechanism of the LNT."
Our news journalists obtained a quote from the research from Sun Yat Sen University, "In a tumor-targeting manner, recombinant expressing plasmids harboring wild-type p53 and Rb were either co-transferred or transferred separately to rabbit hepatic VX2 tumors in a poly-L-lysine-modified hydroxyapatite nanoparticle nanoplex and Lipiodol ® (Guerbet, Villepinte, France) emulsion via the hepatic artery. Subsequent co-expression of p53 and Rb proteins within the treated tumors was investigated by Western blotting and in situ analysis by laser-scanning confocal microscopy. The therapeutic effect was evaluated by the tumor growth velocity, apoptosis and necrosis rates, their sensitivity to Adriamycin ® (ADM), mitomycin C, and fluorouracil, the microvessel density of tumor tissue, and the survival time of animals. Eventually, real-time polymerase chain reaction and enhanced chemiluminescence Western blotting were used to investigate the expressive changes of important genes related to the therapy. The administration procedure proved safe for the rabbits' liver function, the p53 plus Rb LNT showed significantly better antitumoral effect and lower expression of malignant genes than the p53 or Rb LNT, although no significant difference was observed in animal survival when the p53 plus Rb LNT was compared with the p53 LNT. Rb works synergistically with p53 in combined therapy mediated by a poly-L-lysine-modified hydroxyapatite nanoparticle nanoplex to augment the antitumoral effect through the downregulated expression of important genes related to apoptosis, necrosis, growth, differentiation and multidrug resistance of tumor cells."
According to the news editors, the research concluded: "LNT with p53 and Rb is potentially an effective antitumor therapy for hepatocellular carcinoma."
For more information on this research see: The cooperative effect of p53 and Rb in local nanotherapy in a rabbit VX2 model of hepatocellular carcinoma. International Journal of Nanomedicine, 2013;8():3757-3768. International Journal of Nanomedicine can be contacted at: Dove Medical Press Ltd, PO Box 300-008, Albany, Auckland 0752, New Zealand (see also Nanomedicine).
Our news journalists report that additional information may be obtained by contacting S.L. Dong, Sun Yat Sen UniversitySun Yat Sen Mem Hosp, Dept. of Ultrasound, Guangzhou 510120, Guangdong, People's Republic of China. Additional authors for this research include Q.B. Tang, M.Y. Long, J. Guan, L. Ye and G.P. Li.
Keywords for this news article include: Asia, Therapy, Guangdong, Nanomedicine, People's Republic of China
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