By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Nanoparticles is now available. According to news reporting originating from Nanjing, People's Republic of China, by NewsRx correspondents, research stated, "Matriptase, a type II transmembrane serine protease, is responsible for the early stage proliferation of a wide range of human cancers. It is highly expressed on the surface of tumor cells, allowing the protease to serve as a biomarker of cancer detection."
Our news editors obtained a quote from the research from China Pharmaceutical University, "Hence, in this study, we designed two molecular beacons consisting of a fluorescent dye-peptide conjugate attached to gold nanoparticles (Au NPs) for in vitro and in vivo detection of the matriptase expression on tumor cells. The peptide substrate (GRQSRAGC) for matriptase served as the linker between dye (donor) and Au NP (acceptor). In this configuration, the dye fluorescence was quenched by Au NP under physiological conditions and recovered after selective cleavage of the GRQSRAGC by matriptase. To maximize spectral overlap between dye fluorescence and Au NP absorption and optimize the quenching efficiency mediated by fluorescence resonance energy transfer (FRET), the visible dye (fluorescein) and near-infrared (NIR) dye peptide conjugates were each attached to spherical and rod-shaped Au NP for in vitro and in vivo detection, respectively."
According to the news editors, the research concluded: "Both in vitro cell and in vivo animal studies indicate that these two molecular beacons are sensitive and specific for the detection of matriptase expression in the tumor, thereby paving the way to image the activities of this diagnostic protease in living organisms."
For more information on this research see: Gold nanoparticles based molecular beacons for in vitro and in vivo detection of the matriptase expression on tumor. Biosensors & Bioelectronics, 2013;49():216-21. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting D. Deng, Dept. of Biomedical Engineering, China Pharmaceutical University, Nanjing 210009, People's Taiwan. Additional authors for this research include D. Zhang, Y. Li, S. Achilefu and Y. Gu (see also Nanoparticles).
Keywords for this news article include: Asia, Nanjing, Protease, Nanotechnology, Gold Nanoparticles, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC