By a News Reporter-Staff News Editor at Science Letter -- Investigators discuss new findings in Proteobacteria. According to news reporting from Los Angeles, California, by NewsRx journalists, research stated, "Synergistic microbial communities are ubiquitous in nature and exhibit appealing features, such as sophisticated metabolic capabilities and robustness. This has inspired fast-growing interest in engineering synthetic microbial consortia for biotechnology development."
The news correspondents obtained a quote from the research from the University of California, "However, there are relatively few reports of their use in real-world applications, and achieving population stability and regulation has proven to be challenging. In this work, we bridge ecology theory with engineering principles to develop robust synthetic fungal-bacterial consortia for efficient biosynthesis of valuable products from lignocellulosic feedstocks. The required biological functions are divided between two specialists: the fungus Trichoderma reesei, which secretes cellulase enzymes to hydrolyze lignocellulosic biomass into soluble saccharides, and the bacterium Escherichia coli, which metabolizes soluble saccharides into desired products. We developed and experimentally validated a comprehensive mathematical model for T. reesei/E. coli consortia, providing insights on key determinants of the system's performance. To illustrate the bioprocessing potential of this consortium, we demonstrate direct conversion of microcrystalline cellulose and pretreated corn stover to isobutanol. Without costly nutrient supplementation, we achieved titers up to 1.88 g/L and yields up to 62% of theoretical maximum. In addition, we show that cooperator-cheater dynamics within T. reesei/E. coli consortia lead to stable population equilibria and provide a mechanism for tuning composition."
According to the news reporters, the research concluded: "Although we offer isobutanol production as a proof-of-concept application, our modular system could be readily adapted for production of many other valuable biochemicals."
For more information on this research see: Design and characterization of synthetic fungal-bacterial consortia for direct production of isobutanol from cellulosic biomass. Proceedings of the National Academy of Sciences of the United States of America, 2013;110(36):14592-14597. Proceedings of the National Academy of Sciences of the United States of America can be contacted at: Natl Acad Sciences, 2101 Constitution Ave NW, Washington, DC 20418, USA. (National Academy of Sciences - www.nasonline.org/; Proceedings of the National Academy of Sciences of the United States of America - www.nasonline.org/publications/pnas/)
Our news journalists report that additional information may be obtained by contacting J.J. Minty, University of California, Dept. of Chem & Biomol Engn, Los Angeles, CA 90095, United States. Additional authors for this research include M.E. Singer, S.A.Scholz, C.H. Bae, J.H. Ahn, C.E. Foster, J.C. Liao and X.N. Lin (see also Proteobacteria).
Keywords for this news article include: California, Los Angeles, Engineering, United States, Proteobacteria, North and Central America
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