By a News Reporter-Staff News Editor at Science Letter -- Investigators discuss new findings in Proteins. According to news originating from Changsha, People's Republic of China, by NewsRx correspondents, research stated, "In this study, off-line two-dimensional (2D) complexation high speed counter-current chromatography (HSCCC) was developed for the separation of bovine serum albumin (BSA) binders from the ethyl acetate extract of Fructus polygoni orientalis. Target-guided strategy of BSA functionalized iron oxide magnetic nanoparticles coupled with high performance liquid chromatography-tandem mass spectrometry ((BSA-Fe3O4 MNPs)-HPLC-MS/MS) experiment was proposed."
Our news journalists obtained a quote from the research from Central South University, "In the orthogonal separation system, a Normal-Phase HSCCC with 0.01 mol/L copper ion as complexation agent in the aqueous phase was employed for the first dimension and Recycling HSCCC, Reverse-Phase HSCCC with 0.1 mol/L copper ion were used for the second dimension in parallel. Including two pairs of cis-trans isomers, seven BSA binders including 3,5,7-Trihydroxychromone (1), taxifolin (2), N-cis-paprazine (3), N-cis-feruloyltyramine (4), N-trans-paprazine (5), N-trans-feruloyltyramine (6) and an unidentified compound (7) were obtained. The purities of these seven compounds were all over 95.0% as determined by HPLC. The complexation HSCCC behaviors of seven compounds were also investigated by studying their relationship with copper ion. Results showed that the combinative method using (BSA-Fe3O4 MNPs)-HPLC and HSCCC is a quick, efficient, and reproductive technique to isolate potentially bioactive compounds from the complex mixture system of natural products."
According to the news editors, the research concluded: "And the usage of off-line 2D-HSCCC and introduction of chelating metal ion into solvent system are effective ways to implement HSCCC separations in complex samples."
For more information on this research see: Separation and purification of bovine serum albumin binders from Fructus polygoni orientalis using off-line two-dimensional complexation high-speed counter-current chromatography target-guided by ligand fishing. Journal of Chromatography A, 2013;1304():183-193. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
The news correspondents report that additional information may be obtained from Q. Liu, Central South University, Minist Educ, Key Lab Resources Chem Nonferrous Met, Changsha 410083, Hunan, People's Republic of China. Additional authors for this research include S.Y. Shi, L.L. Liu, H. Yang, W. Su and X.Q. Chen (see also Proteins).
Keywords for this news article include: Asia, Changsha, Blood Proteins, Acute-Phase Proteins, Bovine Serum Albumin, People's Republic of China
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