By a News Reporter-Staff News Editor at Science Letter -- Research findings on Proteins are discussed in a new report. According to news reporting originating in Lund, Sweden, by NewsRx journalists, research stated, "Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody."
The news reporters obtained a quote from the research from Lund University, "We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1)."
According to the news reporters, the research concluded: "The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples."
For more information on this research see: Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody. Analytica Chimica Acta, 2013;796():108-114. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
Our news correspondents report that additional information may be obtained by contacting S. Lee, Lund University, Dept. of Elect Measurements, S-22100 Lund, Sweden. Additional authors for this research include S. Kim, J. Malm, O.C. Jeong, H. Lilja and T. Laurell (see also Proteins).
Keywords for this news article include: Lund, Antibodies, Sweden, Europe, Immunology, Kallikreins, Blood Proteins, Endopeptidases, Nanotechnology, Porous Silicon, Immunoglobulins, Serine Proteases, Neoplasm Antigens, Peptide Hydrolases, Emerging Technologies, Biological Tumor Markers, Prostate-Specific Antigen, Prostatic Secretory Proteins
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