By a News Reporter-Staff News Editor at Science Letter -- Investigators discuss new findings in Life Science Research. According to news reporting from Wuhan, People's Republic of China, by NewsRx journalists, research stated, "Somatic embryogenesis (SE) is a most promising technology that is used for in vitro germplasm conservation and genetic improvement via biotechnological approaches in citrus. Herein, three suppression subtractive hybridization (SSH) libraries were constructed using calluses of Citrus sinensis cv. 'Valencia' to explore the molecular mechanisms that underlie the SE in citrus."
The news correspondents obtained a quote from the research from Huazhong Agricultural University, "A total of 880 unisequences were identified by microarray screening based on these three SSH libraries. Gene ontology analysis of the differentially expressed genes indicated that nucleolus associated regulation and biogenesis processes, hormone signal transduction, and stress factors might be involved in SE. Transcription factors might also play an important role. LEC1/B3 domain regulatory network genes (LEC1, L1L, FUS3, ABI3, and ABI5) were isolated in citrus SE. Some new transcription factors associated with citrus SE, like a B3 domain containing gene and HB4, were identified. To understand the influence of these isolated genes on SE competence, their expression profiles were compared among callus lines of seven citrus cultivars with different SE competence. The expression dynamics suggested that these genes could be necessary for the SE initiation and might play a role in embryogenic competence maintenance in different cultivars. On the basis of gene expression profiles, an overview of major physiological and biosynthesis processes at different developmental stages during citrus SE is presented."
According to the news reporters, the research concluded: "For the first time, these data provide a global resource for transcriptional events important for SE in citrus, and the specific genes offer new information for further investigation on citrus SE maintenance and development."
For more information on this research see: Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH-based microarray. Planta, 2012;236(4):1107-24. Planta can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Planta - www.springerlink.com/content/0032-0935/)
Our news journalists report that additional information may be obtained by contacting X.X. Ge, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Taiwan. Additional authors for this research include L.J. Chai, Z. Liu, X.M. Wu, X.X. Deng and W.W Guo (see also Life Science Research).
Publisher contact information for the journal Planta is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Asia, Wuhan, Life Science Research, People's Republic of China.
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