By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Analytical Science. According to news reporting originating in Enschede, Netherlands, by NewsRx journalists, research stated, "Today, droplet based microfluidics has become a standard platform for high-throughput single cell experimentation and analysis. However, until now no label-free, integrated single cell detection and discrimination method in droplets is available."
The news reporters obtained a quote from the research from the University of Twente, "We present here a microfluidic chip for fast (>100 Hz) and label-free electrical impedance based detection of cells in droplets. The microfluidic glass-PDMS device consists of two main components, the droplet generator and the impedance sensor. The planar electrode pair in the main channel allows the detection of only cells and cell containing droplets passing the electrodes using electrical impedance measurements. At a measurement frequency of 100 kHz non-viable cells, in low-conducting (LC) buffer, show an increase in impedance, due to the resistive effect of the membrane. The opposite effect, an impedance decrease, was observed when a viable cell passed the electrode pair, caused by the presence of the conducting cytoplasm. Moreover, we found that the presence of a viable cell in a droplet also decreased the measured electrical impedance. This impedance change was not visible when a droplet containing a non-viable cell or an empty droplet passed the electrode pair. A non-viable cell in a droplet and an empty droplet were equally classified. Hence, droplets containing (viable) cells can be discriminated from empty droplets."
According to the news reporters, the research concluded: "These results provide us with a valuable method to label-free detect and select viable cells in droplets. Furthermore, the proposed method provides the first step towards additional information regarding the encapsulated cells (e.g., size, number, morphology). Moreover, this all-electric approach allows for all-integrated Lab on a Chip (LOC) devices for cell applications using droplet-based platforms."
For more information on this research see: Label-free, high-throughput, electrical detection of cells in droplets. Analyst, 2013;138(16):4585-92. (Royal Society of Chemistry - www.rsc.org/; Analyst - pubs.rsc.org/en/journals/journalissues/an)
Our news correspondents report that additional information may be obtained by contacting E.W. Kemna, BIOS, Lab on a Chip group, MESA? Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, Netherlands. Additional authors for this research include L.I. Segerink, F. Wolbers, I. Vermes and A. van den Berg (see also Analytical Science).
Keywords for this news article include: Europe, Enschede, Netherlands, Analytical Science.
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