By a News Reporter-Staff News Editor at Veterinary Week -- Researchers detail new data in Stem Cell Research. According to news reporting from Chongqing, People's Republic of China, by VerticalNews journalists, research stated, "Epidermal stem cells (ESCs) play an important role in skin homeostasis, wound repair, and tumorigensis which have great potential in scientific research and clinical application. So, the efficient isolation of these infrequent stem cells is very important for researchers to solve the problem of low purity and insufficient quantity of stem cells in vitro."
The news correspondents obtained a quote from the research from Third Military Medical University, "The aim of this study was to investigate a method for the enrichment of ESCs by magnetic activated cell sorting system. The isolation strategy was CD71 depletion followed by alpha 6-integrin positive selection. The percentage of alpha 6(bri)CD71(dim) cells in isolated cells was 94.59%. Transmission electron microscopy results revealed that alpha 6(bri) CD71(dim) cells exhibited some typical characteristics like progenitor cells, such as big nucleus, obvious nucleolus, large nuclear-cytoplasm ratio, and few organelles in cytoplasm. When cultured in vitro, the alpha 6(bri)CD71(dim) cells had greater proliferating potential and higher colony-forming ability, and high levels of epidermal stem cell markers were expressed in our positive cells. ESCs have been successfully isolated from neonatal epidermis using Vario MACS and cultured in vitro."
According to the news reporters, the research concluded: "This isolation method is simple, fast, and inexpensive, providing an important tool for tissue engineering and cell transplantation studies."
For more information on this research see: Enrichment of epidermal stem cells of rats by Vario magnetic activated cell sorting system. In Vitro Cellular & Developmental Biology-Animal, 2013;49(8):583-588. In Vitro Cellular & Developmental Biology-Animal can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA.
Our news journalists report that additional information may be obtained by contacting W. Chen, Third Military Medical University, Dept. of Cell Biol, Chongqing 400038, People's Republic of China. Additional authors for this research include W.W. Zhang, C.Y. Shi, X.H. Lian, S.H. Yi and T. Yang.
Keywords for this news article include: Asia, Tissue Engineering, Biomedical Engineering, Biomedicine, Chongqing, Bioengineering, Stem Cell Research, People's Republic of China
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