By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Researchers detail new data in Enzymes and Coenzymes. According to news reporting originating from Shandong, People's Republic of China, by NewsRx correspondents, research stated, "Antisense oligonucleotides (ASODNs) have been widely used as an important tool for regulating gene expression, and developed into therapeutics. Natural ODNs are susceptible to nuclease degradation, nucleic acid analogues, however, have less side effects, stronger stability and more potent activities."
Our news editors obtained a quote from the research from the Ocean University of China, "Large-scale de novo synthesis of a certain oligonucleotide has been very difficult and costly. In a previous preliminary study, we developed the polymerase-endonuclease amplification reaction (PEAR) for amplification and large-scale preparation of natural antisense ODNs. Here we extended the method in preparation of a widely used modified oligonucleotide with 5'-O-(1-Thiotriphosphate) modifications. Using electrospray ionization liquid chromatography mass spectrometry (ESI/LC/MS) detection, the purity of the PEAR product was measured as high as 100.0%. Using PEAR a large amount of a specific oligonucleotide can be produced starting from a small amount of synthetic seeds."
According to the news editors, the research concluded: "It is suggested that PEAR can be a useful tool for large-scale production of modified oligonucleotides."
For more information on this research see: Preparation of 5'-O-(1-Thiotriphosphate)-modified oligonucleotides using polymerase-endonuclease amplification reaction (PEAR). Plos One, 2013;8(7):e67558. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news editors report that additional information may be obtained by contacting B. Li, Dept. of Biotechnology, Ocean University of China, Qingdao, Shandong, People's Taiwan. Additional authors for this research include S. Dong, J. Wu, J. Zhang, G. Chen, Q. Dong, X. Zhu and X. Wang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Antisense Technology, Biotechnology, Shandong, Esterases, Polymerase, Endonucleases, Bioengineering, Enzymes and Coenzymes, People's Republic of China.
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