By a News Reporter-Staff News Editor at Agriculture Week -- A new study on Enzymes and Coenzymes is now available. According to news originating from Seoul, South Korea, by VerticalNews correspondents, research stated, "Urokinase-type plasminogen activator is a serine protease that is clinically used in humans for the treatment of thrombolytic disorders and vascular diseases such as acute ischemic stroke and acute peripheral arterial occlusion. This study explored the feasibility of using chickens as a bioreactor for producing human urokinase-type plasminogen activator (huPA)."
Our news journalists obtained a quote from the research from Konkuk University, "Recombinant huPA gene, under the control of a ubiquitous Rous sarcoma virus promoter, was injected into the subgerminal cavity of freshly laid chicken eggs at stage X using the replication-defective Moloney murine leukemia virus (MoMLV)-based retrovirus vectors encapsidated with VSV-G (vesicular stomatitis virus G) glycoprotein. A total of 38 chicks, out of 573 virus-injected eggs, hatched and contained the huPA gene in their various body parts. The mRNA transcript of the huPA gene was present in various organs, including blood and egg, and was germ-line transmitted to the next generation. The level of active huPA protein was 16-fold higher in the blood of the transgenic chicken than in the nontransgenic chicken (p
According to the news editors, the research concluded: "Transgenic chickens, expressing the huPA under the control of a ubiquitous promoter, may not only be used as a bioreactor for pharming of the huPA drug but also be useful for studying huPA-induced bleeding and other disorders."
For more information on this research see: Transgenic chickens expressing human urokinase-type plasminogen activator. Poultry Science, 2013;92(9):2396-403. Poultry Science can be contacted at: Poultry Science Assoc Inc, 1111 North Dunlap Ave, Savoy, IL 61874-9604, USA.
The news correspondents report that additional information may be obtained from S.H. Lee, Dept. of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, 1 Hwayang-dong, Gwangjin-Gu, Seoul, 143 701, South Korea. Additional authors for this research include M.K. Gupta, Y.T. Ho, T. Kim and H.T Lee.
The publisher's contact information for the journal Poultry Science is: Poultry Science Assoc Inc, 1111 North Dunlap Ave, Savoy, IL 61874-9604, USA.
Keywords for this news article include: Asia, Seoul, Viruses, Virology, South Korea, Beta Globulins, Blood Proteins, Enzyme Precursors, Peptide Hydrolases, Enzymes and Coenzymes, Serine Endopeptidases, Blood Coagulation Factors, Urokinase Type Plasminogen Activator.
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