By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Immunology are presented in a new report. According to news originating from Clayton, Australia, by NewsRx correspondents, research stated, "Bioactive papers are usually challenged by four major limitations: sensitivity, selectivity, simplicity and strength (4S). Gold nanoparticles (AuNPs) treated paper has previously been demonstrated as a Surface Enhanced Raman Scattering (SERS) active substrate, capable of addressing the 4S issues."
Our news journalists obtained a quote from the research from Monash University, "In this study, AuNPs on paper substrate were functionalized by a series of biomolecules to develop a generic SEAS platform for antibody-antigen detection. The functionalization steps were performed by taking advantage of the high affinity association between Streptomyces avidinii-derived protein, streptavidin, and biotin. Streptavidin was firstly bound onto the AuNPs treated paper using biotinylated-thiol. Subsequently, desired biotinylated-antibody was bound onto the streptavidin. SERS spectra of each functionalization step were obtained to ensure specific adsorption of the bio-molecules. The binding interaction of the antibody with its specific antigen was detected using SEAS. Shifts of Raman band associated with a-helix and beta-sheet structures indicated structural modification of the antibody upon interaction with its antigen. Predominant tryptophan and tyrosine residue bands were also detected, confirming the presence of antigen. Reproducible spectral features were quantified as AuNP papers were subjected to different concentrations of antigen; the spectra intensity increased as a function of the antigen concentration. The retention of AuNPs on paper remained constant after all the consecutive washing and functionalization steps."
According to the news editors, the research concluded: "The feasibility of AuNPs paper as a low-cost and generic SERS platform for bio-diagnostic applications was demonstrated."
For more information on this research see: Gold nanoparticles paper as a SERS bio-diagnostic platform. Journal of Colloid and Interface Science, 2013;409():59-65. Journal of Colloid and Interface Science can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Journal of Colloid and Interface Science - www.elsevier.com/wps/product/cws_home/622861)
The news correspondents report that additional information may be obtained from Y.H. Ngo, Monash University, Dept. of Chem Engn, Australian Pulp & Paper Inst APPI, BioPRIA, Clayton, Vic 3800, Australia. Additional authors for this research include W.L. Then, W. Shen and G. Garnier (see also Immunology).
Keywords for this news article include: Antibodies, Clayton, Immunology, Streptavidin, Blood Proteins, Nanotechnology, Immunoglobulins, Bacterial Proteins, Gold Nanoparticles, Emerging Technologies, Australia and New Zealand
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