By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on Oncology have been published. According to news reporting out of Hefei, People's Republic of China, by NewsRx editors, research stated, "NOB1 (NIN1/RPN12 binding protein 1 homolog), a ribosome assembly factor, is thought to be essential for the processing of the 20S pre-rRNA into the mature 18S rRNA. It is also reported to participate in proteasome biogenesis."
Our news journalists obtained a quote from the research from Anhui Medical University, "However, the contribution of NOB1 gene dysfunction to the pathology of human diseases, such as gliomas, has not been addressed. Here, we detected expression levels of NOB1 mRNA in U251, U87, U373, and A172 cells by quantitative real-time PCR. To analyze the expression levels of NOB1 protein in glioma tissues, we performed immunohistochemistry on 56 pathologically confirmed glioma samples (7 Grade I cases, 19 Grade II cases, 16 Grade III cases, and 14 Grade IV cases). A recombinant lentivirus expressing NOB1 short hairpin RNA (shNOB1) was constructed and infected into 13251 and U87-MG human glioma cells. We found that NOB1 mRNA was expressed in all four cell lines. The expression level of the NOB1 protein was significantly higher in high-grade gliomas than in low-grade gliomas. Knockdown of the NOB1 gene resulted in suppression of the proliferation and the colony-forming abilities of U251 and U87-MG cells, cell cycle arrest during the G(0)/G(1) phase, and a significant enhancement of cell apoptosis. In addition, cell migration was significantly suppressed in 13251 and U87-MG cells that were infected with the shNOB1-expressing lentivirus."
According to the news editors, the research concluded: "These results suggest that NOB1 promotes glioma cell growth and migration and could be a candidate for molecular targeting during gene therapy treatments of glioma."
For more information on this research see: Knockdown of NOB1 expression by RNAi inhibits cellular proliferation and migration in human gliomas. Gene, 2013;528(2):146-153. Gene can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Gene - www.elsevier.com/wps/product/cws_home/506033)
Our news journalists report that additional information may be obtained by contacting H.L. Wang, Anhui Medical University, Affiliated Hosp 2, Dept. of Neurosurg, Hefei 230601, People's Republic of China. Additional authors for this research include P. Li and B. Zhao (see also Oncology).
Keywords for this news article include: Asia, Biotechnology, Hefei, Glioma, Oncology, Gene Therapy, Bioengineering, People's Republic of China
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