By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting out of Faisalabad, Pakistan, by NewsRx editors, research stated, "Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692 +/- 12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation."
Our news journalists obtained a quote from the research from the Faisalabad University of Agriculture, "The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH 4 and 70 degrees C, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants K-M and V-max. To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period."
According to the news editors, the research concluded: "A lower K-M, higher V-max, hyper-activation, and enhanced acidic and thermal resistance up to 70 degrees C were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes."
For more information on this research see: Novel catalytic and effluent decolorization functionalities of sol-gel immobilized Pleurotus ostreatus IBL-02 manganese peroxidase produced from bio-processing of wheat straw. Chinese Journal of Catalysis, 2013;34(9):1756-1761. Chinese Journal of Catalysis can be contacted at: Science Press, 16 Donghuangchenggen North St, Beijing 100717, Peoples R China. (Elsevier - www.elsevier.com; Chinese Journal of Catalysis - www.elsevier.com/wps/product/cws_home/709300)
Our news journalists report that additional information may be obtained by contacting M. Asgher, Faisalabad University of Agriculture, Dept. of Chem & Biochem, Ind Biotechnol Lab, Faisalabad, Pakistan. Additional authors for this research include B. Aslam and H.M.N. Iqbal (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Pakistan, Faisalabad, Peroxidases, Oxidoreductases, Enzymes and Coenzymes
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