By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Research findings on Biotechnology are discussed in a new report. According to news originating from Changchun, People's Republic of China, by NewsRx correspondents, research stated, "A novel antisense oligonucleotide (ASO) carrier, polyethylenimine conjugated to linoleic acid (PEI-LA), was synthesized and evaluated for delivery of LOR-2501 to tumor cells. LOR-2501 is an ASO targeting ribonucleotide reductase R1 subunit (RRM1)."
Our news journalists obtained a quote from the research from Jilin University, "In this study, PEI-LA was synthesized by reacting PEI (Mw ~ 800) with linoleoyl chloride. Gel retardation assay showed complete complexation between PEI-LA and LOR-2501 at N/P ratio above 8. No significant cytotoxicity was observed with these complexes at the tested dosage levels. Interestingly, at N/P ratio of >6, levels of cellular uptake of PEI-LA/LOR-2501 were double that of PEI/LOR-2501 complexes of the same N/P ratio. PEI-LA/LOR-2501 induced downregulation of 64% and 70% of RRM1 at mRNA and protein levels, respectively. The highest transfection activity was shown by PEI-LA/LOR-2501 complexes at N/P ratio of 10. Finally, using pathway specific inhibitors, clathrin-mediated endocytosis was shown to be the principle mechanism of cellular internalization of these complexes."
According to the news editors, the research concluded: "PEI-LA is a promising agent for the delivery of ASOs and warrants further investigation."
For more information on this research see: A polyethylenimine-linoleic acid conjugate for antisense oligonucleotide delivery. Biomed Research International, 2013;2013():710502 (see also technology.html">Biotechnology).
The news correspondents report that additional information may be obtained from J. Xie, Institute of Life Sciences, Jilin University, Changchun, Jilin 130023, People's Taiwan. Additional authors for this research include L. Teng, Z. Yang, C. Zhou, Y. Liu, B.C. Yung and R.J Lee.
Keywords for this news article include: Asia, Antisense Technology, Biotechnology, Changchun, Bioengineering, People's Republic of China.
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