By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Gram-Positive Bacteria. According to news reporting originating in Fukuoka, Japan, by NewsRx journalists, research stated, "We previously reported a butanol production process with pH-stat continuous feeding of dl-lactic acid and glucose as the co-substrate (Oshiro et al., Appl. Microbiol. Biotechnol., 87, 1177-1185, 2010)."
The news reporters obtained a quote from the research from Kyushu University, "To accomplish butanol production from completely inedible substrates, in this study, we investigated acetone-butanol-ethanol (ABE) fermentation of Clostridium saccharoperbutylacetonicum N1-4 with lactic acid by using pentose as the co-substrate. Examination for optimum co-substrate indicated that arabinose was superior to glucose and xylose for ABE fermentation. Actually batch culture with lactic acid and arabinose without pH control exhibited higher butanol production (7.11 g/l) and lactic acid consumption (2.02 g) than those (6.62 g/l and 1.45 g, respectively) with glucose. Fed-batch culture without pH control increased these values to 12.08 g/l and 15.60 g/l butanol production, and to 3.83 g and 5.91 g lactic acid consumption by feeding the substrate once and twice, respectively. Finally, the result of gas chromatography-mass spectroscopy analysis using [1,2,3-(13)C(3)]-lactic acid indicated that lactic acid was converted to butanol with the efficiency of 51.9%."
According to the news reporters, the research concluded: "Thus, we established a novel high butanol production from lactic acid using arabinose as the co-substrate in simple fed-batch culture."
For more information on this research see: Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum. Journal of Bioscience and Bioengineering, 2012;114(5):526-30. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
Our news correspondents report that additional information may be obtained by contacting T. Yoshida, Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Dept. of Bioscience and Biotechnology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Additional authors for this research include Y. Tashiro and K. Sonomoto (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Japan, Fukuoka, Lactates, Clostridium, Lactic Acid, Gram-Positive Bacteria, Gram Positive Endospore Forming Rods, Gram Positive Endospore Forming Bacteria.
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