By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Analytical Chemistry. According to news reporting originating in Ithaca, New York, by NewsRx journalists, research stated, "A new method is presented to separate, enrich, and sort membrane-bound biomolecules based on their affinity for different coexisting lipid phases in a supported lipid bilayer using a two-dimensional, continuous extraction procedure. Analogous to classic liquid-liquid phase extraction, we created two distinct lipid phases in our planar membrane system: a liquid-ordered (l(o)) phase and a liquid-disordered (l(d)) phase arranged in parallel stripes inside a microfluidic device."
The news reporters obtained a quote from the research from Cornell University, "Membrane-bound biomolecules in an adjacent supported lipid bilayer are convected in plane along the microfluidic channel and brought into contact with a different lipid phase using hydrodynamic force. A mixture of two lipid species, a glycolipid and a phospholipid, with known affinities for the two lipid phases employed here are used to demonstrate continuous extraction of the lipid-microdomain preferring glycolipid to the lo phase, while the phospholipid remains primarily in the ld phase. In this demonstration, we characterize the performance of this affinity-based separation device by building models to describe the velocity profile and transport in the two-phase coexistent membrane. We then characterize the impact of residence time on the extraction yield of each species. This new procedure sorts membrane species on the basis of chemical properties and affinities for specific lipid phases within a membrane environment near physiological conditions, critical for extending this method to the separation of lipid-linked proteins and transmembrane proteins while minimizing denaturation."
According to the news reporters, the research concluded: "This platform could facilitate the separation and identification of lipid membrane domain residents, or the characterization of changes in membrane affinity due to post-translational modifications or environmental conditions."
For more information on this research see: Two-dimensional continuous extraction in multiphase lipid bilayers to separate, enrich, and sort membrane-bound species. Analytical Chemistry, 2013;85(14):6696-702. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news correspondents report that additional information may be obtained by contacting L. Chao, School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, United States. Additional authors for this research include M.J. Richards, C.Y. Hsia and S. Daniel (see also Analytical Chemistry).
Keywords for this news article include: Ithaca, New York, United States, Analytical Chemistry, North and Central America.
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