By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteins. According to news reporting originating in Garching, Germany, by NewsRx journalists, research stated, "There is great interest in establishing efficient means of organizing nanoparticles into complex structures, especially in fields like nano-optical devices. One of the demonstrated routes uses biomolecular scaffolds, like the streptavidin-biotin system, to deterministically separate and structure particle complexes."
The news reporters obtained a quote from the research from Technical University, "However, controlled formation of streptavidin-linked nanoparticle dimers or trimers is challenging, and large aggregates are often formed under conditions that are difficult to regulate. Here, we studied the aggregates and interlinking kinetics of biotin-functionalized 20 nm gold nanoparticles in the presence of the interlinking protein, streptavidin. We found two different protein-linker concentration regions where small stable particle aggregates are formed: when the protein and nanoparticle concentrations are similar and when the protein to nanoparticle concentration ratio exceeds intermediate concentrations (10:1-100:1) that promote precipitation of large aggregates. We attribute this behavior to the limited availability of free-linker molecules and the limited availability of free ligand (biotin) on the particle surface for low and high protein concentrations, respectively. Furthermore, we show that the product can be additionally enriched up to 25 % through either centrifugation in sucrose or size-exclusion chromatography."
According to the news reporters, the research concluded: "These results provide additional understanding into the assembly of ligand-functionalized nanoparticles with water-soluble linkers and provide a facile way to produce well-defined small aggregates for potential use in, for instance, surface-enhanced spectroscopy."
For more information on this research see: Preparation of gold nanoparticle dimers via streptavidin-induced interlinking. Journal of Nanoparticle Research, 2013;15(10):1-10. Journal of Nanoparticle Research can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Journal of Nanoparticle Research - www.springerlink.com/content/1388-0764/)
Our news correspondents report that additional information may be obtained by contacting V.B. Zon, Technical University of Munich, Walter Schottky Inst, D-85748 Garching, Germany. Additional authors for this research include M. Sachsenhauser and U. Rant (see also Proteins).
Keywords for this news article include: Europe, Germany, Garching, Streptavidin, Nanotechnology, Bacterial Proteins, Gold Nanoparticles, Emerging Technologies
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