By a News Reporter-Staff News Editor at Gastroenterology Week -- Fresh data on Proteins are presented in a new report. According to news reporting originating from La Jolla, California, by NewsRx correspondents, research stated, "To develop a mouse model for multispectral fluorescence imaging of the pancreas and pancreatic microenvironment. Cre/loxP technology was used to develop this model."
Our news editors obtained a quote from the research from Moores Cancer Center, "We crossed mT/mG indicator mice, engineered to constitutively express a conditional tdTomato transgene that converts to green fluorescent protein (GFP) expression after exposure to Cre recombinase, with Pdx1-Cre transgenic mice. To characterize this model for studies of pancreas biology, we performed bright light and fluorescence imaging of body cavities and intact organs and confocal microscopy of pancreata from offspring of Pdx1-Cre and mT/mG crosses. Pdx1-Cre-mT/mG mice demonstrated bright GFP expression within the pancreas and duodenum and intense tdTomato expression in all other organs. Green fluorescent protein expression was mosaic in Pdx1-Cre-mT/mG pancreata, with most showing extensive conversion from tdTomato to GFP expression within the epithelial-derived elements of the pancreatic parenchyma. Because both GFP and tdTomato are membrane targeted, individual cell borders were clearly outlined in confocal images of mT/mG pancreata."
According to the news editors, the research concluded: "This mouse model enables multispectral fluorescence imaging of individual cells and cell processes at the microscopic level of the pancreatic microenvironment; it should prove valuable for a variety of fluorescence imaging studies, ranging from pancreatic development to pancreatic cancer biology."
For more information on this research see: A dual-color genetically engineered mouse model for multispectral imaging of the pancreatic microenvironment. Pancreas, 2013;42(6):952-8. (Lippincott Williams and Wilkins - www.lww.com; Pancreas - journals.lww.com/pancreasjournal/pages/default.aspx)
The news editors report that additional information may be obtained by contacting C.S. Snyder, Dept. of Surgery, UCSD Moores Cancer Center, La Jolla, CA 92093-0987, United States. Additional authors for this research include A.R. Harrington, S. Kaushal, E. Mose, A.M. Lowy, R.M. Hoffman and M. Bouvet (see also Proteins).
Keywords for this news article include: La Jolla, Pancreas, California, Technology, United States, Bioengineering, Gastroenterology, Genetic Engineering, North and Central America, Green Fluorescent Protein.
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