By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news originating from Kharagpur, India, by NewsRx correspondents, research stated, "Betacyanins are the major pigments present in Amaranthus tricolor, a leafy vegetable consumed globally. The terminal glycosyltaion of the aglycone betanidin is an important step in the biosynthesis of this natural red antioxidant pigment."
Our news journalists obtained a quote from the research from the Indian Institute of Technology, "A betanidin 5-O-glucosyltransferase (BGT) was fully purified to 134 folds (specific activity, 265.2 nkat mg(-1)) from the red amaranth by ammonium sulfate precipitation followed by hydrophobic interaction, anion exchange and size exclusion chromatography. Homogeneity of the purified protein was confirmed by 2-dimensional polyacrylamide gel electrophoresis (2D PAGE). The molecular weight of the enzyme determined by liquid chromatography-mass spectrometry (LC-MS) was found to be 62.8 kDa. Furthermore, the enzyme glycosylated flavonoids (kaempferol and quercetin) but not anthocyanidins, presence of which is mutually exclusive to betacyanin accumulating plants. The apparent K-m (344 +/- 234 mu M) and V-max (17.24 mu M min(-1)) of the enzyme were determined by LC-MS/MS. Peptide mass fingerprinting of the purified protein showed 38.4% coverage of peptide masses with anthocyanidin 3-O-glucosyltransferase from Zea mays."
According to the news editors, the research concluded: "Study on this purified enzyme, for the first time, revealed its role of glycosylation in biosynthesis of betacyanin in A. tricolor and indicates promiscuous substrate-specificity."
For more information on this research see: Purification and characterization of a betanidin glucosyltransferase from Amaranthus tricolor L catalyzing non-specific biotransformation of flavonoids. Plant Science, 2013;211():61-69. Plant Science can be contacted at: Elsevier Ireland Ltd, Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland. (Elsevier - www.elsevier.com; Plant Science - www.elsevier.com/wps/product/cws_home/506030)
The news correspondents report that additional information may be obtained from S.S. Das, Indian Inst Technol, Dept. of Biotechnol, Plant Biotechnol Lab, Kharagpur 721302, W Bengal, India. Additional authors for this research include S.S. Gauri, B.B. Misra, M. Biswas and S. Dey (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, Kharagpur, Betalains, Amino Acids, Betacyanins, Hexosyltransferases, Glucosyltransferases, Enzymes and Coenzymes
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