By a News Reporter-Staff News Editor at Biotech Business Week -- Current study results on Fibroblasts have been published. According to news reporting out of Yangling, People's Republic of China, by NewsRx editors, research stated, "This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were analyzed."
Our news journalists obtained a quote from the research from Northwest A&F University, "The results suggested that cell lines with a longer lifespan had stable chromosomes compared with those of cells lines with a shorter lifespan. According to the proliferative lifespan, we divided GFF cell lines into two groups: cell lines with a long lifespan (GFF1/2/7/8/9; group L) and cell lines with a short lifespan (GFF3/4/5/6; group S). Next, a hLYZ expression vector was introduced into these cell lines by electroporation. The efficiencies of colony formation, expansion in culture, and the quality of transgenic clonal cell lines were significant higher in group L than those in group S. The mean fusion rate and blastocyst rate in group L were higher than those in group S (80.3 +/- A 1.7 vs. 65.1 +/- A 4.2 % and 19.5 +/- A 0.6 vs. 15.1 +/- A 1.1 %, respectively, P< 0.05). After transferring cloned embryos into the oviducts of recipient goats, three live kids were born. PCR and Southern blot analyses confirmed integration of the transgene in cloned goats."
According to the news editors, the research concluded: "The lifespan of GFF cell lines has a major effect on the efficiency to produce transgenic cloned goats. Therefore, the proliferative lifespan of primary cells may be used as a criterion to characterize the quality of cell lines for genetic modification and SCNT."
For more information on this research see: Establishment and characterization of fetal fibroblast cell lines for generating human lysozyme transgenic goats by somatic cell nuclear transfer. Transgenic Research, 2013;22(5):893-903. Transgenic Research can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Transgenic Research - www.springerlink.com/content/0962-8819/)
Our news journalists report that additional information may be obtained by contacting J. Liu, Northwest A&F Univ, Minist Agr, Key Lab Anim Biotechnol, Yangling 712100, Shaanxi, People's Republic of China. Additional authors for this research include Y. Luo, L.M. Zheng, Q.Q. Liu, Z.C. Yang, Y.S. Wang, J.M. Su, F.S. Quan and Y. Zhang (see also Fibroblasts).
Keywords for this news article include: Asia, Yangling, Lysozyme, Fibroblasts, Connective Tissue Cells, People's Republic of China
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC