By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Biotechnology. According to news reporting out of Lanzhou, People's Republic of China, by NewsRx editors, research stated, "Polymeric gene-delivery vectors to achieve lack of toxicity and a balance between protection and DNA release remains a formidable challenge. Incorporating intracellular environment-responsive degradable bonds is an appreciable step toward developing safer transfection agents."
Our news journalists obtained a quote from the research from Lanzhou University, "In this study, novel, dual-degradable polycation copolymers (Pluronic-diacrylate [PA]-polyethyleneimine [PEI]-SS) were synthesized through the addition of low molecular weight (800 Da) PEI cross-linked with SS (PEI-SS) to PA. Three PA-PEI-SS copolymers (PA-PEI-SS1, 2, and 3) with different PEI-SS to Pluronic molar ratios were investigated and found to strongly condense plasmid DNA into positively charged nanoparticles with an average particle size of approximately 200 nm and to possess higher stability against DNase I digestion and sodium heparin. Disulfide and ester bonds of the copolymers were susceptible to intracellular redox conditions. In vitro experiments demonstrated that the PA-PEI-SS copolymers had significantly lower cytotoxicity and higher transfection efficiency in both BGC-823 and 293T cell lines than the controls of degradable PEI-SS and nondegradable 25 kDa PEI. Transfection activity was influenced by the PEI-SS content in the polymers and PA-PEI-SS1 showed the highest efficiency of the three copolymers."
According to the news editors, the research concluded: "These studies suggest that these dual-degradable copolymers could be used as potential biocompatible gene delivery carriers."
For more information on this research see: Dual-degradable disulfide-containing PEI-Pluronic/DNA polyplexes: transfection efficiency and balancing protection and DNA release. International Journal of Nanomedicine, 2013;8():3689-3701. International Journal of Nanomedicine can be contacted at: Dove Medical Press Ltd, PO Box 300-008, Albany, Auckland 0752, New Zealand (see also technology.html">Biotechnology).
Our news journalists report that additional information may be obtained by contacting L.F. Zhang, Lanzhou University, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem EngnInst Biochem Engn & Environ, Lanzhou 730000, People's Republic of China. Additional authors for this research include Z.Z. Chen and Y.F. Li.
Keywords for this news article include: Asia, Ions, Biotechnology, Lanzhou, Disulfides, DNA Research, Electrolytes, Gene Therapy, Bioengineering, Inorganic Chemicals, People's Republic of China
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