By a News Reporter-Staff News Editor at China Weekly News -- Data detailed on Biotechnology have been presented. According to news reporting originating from Ottawa, Canada, by VerticalNews correspondents, research stated, "Protection against the avian influenza (AI) H5N1 virus is suspected to be mainly conferred by the presence of antibodies directed against the hemagglutinin (HA) protein of the virus. A single electroporation delivery of 100 or 250 ?g of a DNA vaccine construct, pCAG-HA, carrying the HA gene of strain A/Hanoi/30408/2005 (H5N1), in chickens led to the development of anti-HA antibody response in 16 of 17 immunized birds, as measured by a hemagglutination inhibition (HI) test, competitive enzyme-linked immunosorbent assay (cELISA), and an indirect ELISA."
Our news editors obtained a quote from the research from Canadian Food Inspection Agency, "Birds vaccinated by electroporation (n=11) were protected from experimental AI challenge with strain A/chicken/Pennsylvania/1370/1/1983 (H5N2) as judged by low viral load, absence of clinical symptoms, and absence of mortality (n=11). In contrast, only two out of 10 birds vaccinated with the same vaccine dose (100 or 250 ?g) but without electroporation developed antibodies. These birds showed high viral loads and significant morbidity and mortality after the challenge. Seroconversion was reduced in birds electroporated with a low vaccine dose (10 ?g), but the antibody-positive birds were protected against virus challenge. Nonelectroporation delivery of a low-dose vaccine did not result in seroconversion, and the birds were as susceptible as those in the control groups that received the control pCAG vector. Electroporation delivery of the DNA vaccine led to enhanced antibody responses and to protection against the AI virus challenge."
According to the news editors, the research concluded: "The HI test, cELISA, or indirect ELISA for anti-H5 antibodies might serve as a good predictor of the potency and efficacy of a DNA immunization strategy against AI in chickens."
For more information on this research see: A single electroporation delivery of a DNA vaccine containing the hemagglutinin gene of Asian H5N1 avian influenza virus generated a protective antibody response in chickens against a North American virus strain. Clinical and Vaccine Immunology, 2013;20(4):491-500. (American Society for Microbiology - www.asm.org; Clinical and Vaccine Immunology - cdli.asm.org)
The news editors report that additional information may be obtained by contacting O. Ogunremi, Ottawa Laboratory (Fallowfield), Canadian Food Inspection Agency, Ottawa, Ontario, Canada. Additional authors for this research include J. Pasick, G.P. Kobinger, D. Hannaman, Y. Berhane, A. Clavijo and S. van Drunen Littel-van den Hurk.
Keywords for this news article include: Asia, Antibodies, Biotechnology, Ottawa, Canada, Ontario, Virology, Viral DNA, Immunology, RNA Viruses, DNA Research, DNA Vaccines, Blood Proteins, Immunoglobulins, Orthomyxoviridae, Synthetic Vaccines, Vertebrate Viruses, North and Central America, Influenza A Virus Subtype H5N1.
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