By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteobacteria are discussed in a new report. According to news reporting from Qingdao, People's Republic of China, by NewsRx journalists, research stated, "Xylonate is a valuable chemical for versatile applications. Although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far."
The news correspondents obtained a quote from the research from the Qingdao Institute of Bioenergy and Bioprocess Technology, "In this study, the industrially important microorganism Escherichia coli was engineered to produce xylonate from xylose. Through the coexpression of a xylose dehydrogenase (xdh) and a xylonolactonase (xylC) from Caulobacter crescentus, the recombinant strain could convert 1 g/L xylose to 0.84 g/L xylonate and 0.10 g/L xylonolactone after being induced for 12 h. Furthermore, the competitive pathway for xylose catabolism in E. coli was blocked by disrupting two genes (xylA and xylB) encoding xylose isomerase and xylulose kinase. Under fed-batch conditions, the finally engineered strain produced up to 27.3 g/L xylonate and 1.7 g/L xylonolactone from 30 g/L xylose, about 88% of the theoretical yield."
According to the news reporters, the research concluded: "These results suggest that the engineered E. coli strain has a promising perspective for large-scale production of xylonate."
For more information on this research see: Metabolic engineering of Escherichia coli for the production of xylonate. Plos One, 2013;8(7):e67305. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting Y. Cao, Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, People's Taiwan. Additional authors for this research include M. Xian, H. Zou and H. Zhang (see also Proteobacteria).
Keywords for this news article include: Asia, Qingdao, Proteobacteria, Escherichia coli, Enterobacteriaceae, Metabolic Engineering, Gram Negative Bacteria, People's Republic of China.
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