By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Bioscience and Bioengineering. According to news reporting out of Lethbridge, Canada, by NewsRx editors, research stated, "Constructs with sucrose-sucrose 1-fructosyltransferase (1-SST) from rye and or sucrose-fructan 6-fructosyltransferase (6-SFT) from wheat were placed under the control of wheat aleurone-specific promoter and expressed in triticale using biolistic and microspore transformation. Transgenic lines expressing one or both the 1-SST and the 6-SFT accumulated 50% less starch and 10-20 times more fructan, particularly 6-kestose, in the dry seed compared to the untransformed wild-type (WT) triticale; other fructans ranged in size from DP 4 to DP 15."
Our news journalists obtained a quote from the research from Agriculture and Agri-Food Canada, "During germination from 1 to 4 days after imbibition (dai), fructans were rapidly metabolized and only in transgenic lines expressing both 1-SST and 6-SFT were fructan contents significantly higher than in the untransformed controls after 4 days. In situ hybridization confirmed expression of 6-SFT in the aleurone layer in imbibed seeds of transformed plants. When transgenic lines were subjected to a cold stress of 4°C for 2 days, synthesis of fructan increased compared to untransformed controls during low-temperature germination. The increase of fructan in dry seed and germinating seedling was generally associated with transcript expression levels in transformed plants but total gene expression was not necessarily correlated with the time course accumulation of fructan during germination."
According to the news editors, the research concluded: "This is the first report of transgenic modification of cereals to achieve production of fructans in cereal seeds and during seed germination."
For more information on this research see: Carbohydrate profiling in seeds and seedlings of transgenic triticale modified in the expression of sucrose:sucrose-1-fructosyltransferase (1-SST) and sucrose:fructan-6-fructosyltransferase (6-SFT). Journal of Bioscience and Bioengineering, 2012;114(4):371-8. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
Our news journalists report that additional information may be obtained by contacting C. Diedhiou, Agriculture and Agri-Food Canada Research Centre, 5403 1st Avenue South, Lethbridge, Alberta T1J 4B1, Canada. Additional authors for this research include D. Gaudet, Y. Liang, J. Sun, Z.X. Lu, F. Eudes and A. Laroche (see also Bioscience and Bioengineering).
Keywords for this news article include: Canada, Alberta, Lethbridge, North and Central America, Bioscience and Bioengineering.
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