By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting originating in Hamilton, Canada, by NewsRx editors, the research stated, "The efficient decomposition of biomass into carbohydrates for the sustainable generation of biofuels has become the focus of much research. Yet, limited understanding exists on how the enzymes that catalyze the biochemical conversion of biomass, such as cellulases, interact with cellulose microfibrils and how cellulose structure is changed by cellulolytic enzymes."
The news reporters obtained a quote from the research from McMaster University, "This has spurred the application of high-resolution imaging techniques, such as atomic force microscopy or fluorescence microscopy, to visualize the biomolecular interactions and structural changes that occur at the micro/nanoscale. In particular, fluorescence microscopy offers advantages such as high sensitivity and the ability to monitor species under biologically relevant conditions. Furthermore, the introduction of techniques, such as single molecule or super-resolution fluorescence microscopy, has allowed imaging biomolecules and macromolecular structures with near molecular resolution. These advantages make fluorescence microscopy ideally suited for the study of cell wall structure and cellulose-cellulase interactions. The application of fluorescence microscopy has already yielded key insights into the arrangement of structural polysaccharides in the plant cell wall, the reversibility and binding kinetics of cellulases, their molecular motion on crystalline cellulose, and the structural changes that occur as cellulose is depolymerized by cellulases. Yet, the application of fluorescence to study cellulose-cellulase interactions remains limited."
According to the news reporters, the research concluded: "This review aims at (1) providing an overview of fluorescence microscopy techniques suitable for the study of cellulose-cellulase interactions; (2) the applications of these techniques to date and the key insights obtained; and (3) the opportunities for future studies of the interaction of cell wall degrading enzymes with cellulosic materials."
For more information on this research see: The study of cell wall structure and cellulose-cellulase interactions through fluorescence microscopy. Cellulose, 2013;20(5):2291-2309. Cellulose can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Cellulose - www.springerlink.com/content/0969-0239/)
Our news correspondents report that additional information may be obtained by contacting J.M. Moran-Mirabal, McMaster University, Dept. of Chem & Chem Biol, Hamilton, ON L8S 4M1, Canada (see also Enzymes and Coenzymes).
Keywords for this news article include: Canada, Ontario, Hamilton, Cell Wall, Cellulases, Glucosidases, Cellular Structures, Glycoside Hydrolases, Enzymes and Coenzymes, North and Central America
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