By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteobacteria. According to news originating from Rome, Italy, by NewsRx correspondents, research stated, "Pseudomonas aeruginosa pathogenic potential is controlled via multiple regulatory pathways, including three quorum sensing (QS) systems. LasR is a key QS signal receptor since it acts as a global transcriptional regulator required for optimal expression of main virulence factors. P. aeruginosa modulates the QS response by integrating this cell density-dependent circuit to environmental and metabolic cues."
Our news journalists obtained a quote from the research from the University of Rome, "Hence, QS also controls the adaptation to challenging environmental niches, such as infection sites. However, little is known about the molecular mechanisms connecting QS and other signalling pathways. In this work, DNA-affinity chromatography was used to identify new lasR transcriptional regulators. This approach led to the identification and functional characterization of the TetR-like transcriptional repressor PA3699. This protein was purified and shown to directly bind to the lasR promoter region in vitro. The induction of PA3699 expression in P. aeruginosa PAO1 cultures repressed lasR promoter activity and the production of LasR-dependent virulence factors, such as elastase, pyocyanin, and proteases."
According to the news editors, the research concluded: "These findings suggest a role for PA3699 in P. aeruginosa pathogenicity. P. aeruginosa genome encodes at least 38 TetR-family proteins, and PA3699 is the eighth member of this group functionally characterized so far and the first one shown to bind the lasR promoter in vitro."
For more information on this research see: A new transcriptional repressor of the pseudomonas aeruginosa quorum sensing receptor gene lasR. Plos One, 2013;8(7):e69554. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news correspondents report that additional information may be obtained from F. Longo, Dept. of Sciences, University Roma Tre, Rome, Italy. Additional authors for this research include G. Rampioni, R. Bondi, F. Imperi, G.M. Fimia, P. Visca, E. Zennaro and L. Leoni (see also Proteobacteria).
Keywords for this news article include: Rome, Italy, Europe, Proteobacteria, Pseudomonadaceae, Gram Negative Bacteria, Pseudomonas aeruginosa, Gram Negative Aerobic Bacteria, Gram Negative Aerobic Rods and Cocci.
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