By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Enzymes and Coenzymes have been published. According to news reporting out of Vicosa, Brazil, by NewsRx editors, research stated, "Inactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector)."
Our news journalists obtained a quote from the research from Federal University, "This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum."
According to the news editors, the research concluded: "The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production."
For more information on this research see: Overproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene. Applied Biochemistry and Biotechnology, 2013;169(6):1965-77. Applied Biochemistry and Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
Our news journalists report that additional information may be obtained by contacting J.A. Teixeira, Departamento de Microbiologia, BIOAGRO, Universidade Federal de Vicosa, Vicosa, Minas Gerais, Brazil. Additional authors for this research include J.B. Ribeiro, D.B. Goncalves, M.V. de Queiroz and E.F de Araujo (see also Enzymes and Coenzymes).
Publisher contact information for the journal Applied Biochemistry and Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Vicosa, Brazil, South America, Polygalacturonase, Glycoside Hydrolases, Enzymes and Coenzymes.
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