Florida in the Area of Intercellular Signaling Peptides and Proteins Described -->
By a News Reporter-Staff News Editor at Proteomics Weekly -- Current study results on Intercellular Signaling Peptides and Proteins have been published. According to news originating from Gainesville, Florida, by NewsRx correspondents, research stated, "Platelet-derived growth factors (PDGFs) are important biochemical mediators regulating many physiological and pathophysiological processes, including promotion of the chemotactic recruitment and proliferation of cells involved in wound repair. Previously, homodimers of rhPDGF-AA protein were purified from Escherichia call."
Our news journalists obtained a quote from the research from the University of Florida, "However, eukaryotic proteins often contain posttranslational modifications, such as glycosylation, that are required for biological functions. In this study, an efficient method was established to purify a glycosylated rhPDGF-AA dimer from P. pastoris culture media by one step CM Sepharose ion exchange chromatography yielding about 20 mg/L of over 95% highly purified rhPDGF-AA. Mass spectrometry analysis of the purified rhPDGF-AA displayed a molecular weight (MW) of 27,825.513 Da, composed of a subunit with MW of 15,042.945 Da and a subunit with MW of 12,904.374 Da. The size difference is accounted for by differential glycosylation of the monomers. Biological activity of the rhPDGF-AA was confirmed by its ability to induce NIH/3T3 cells proliferation."
According to the news editors, the research concluded: "The experimental procedure we have developed facilitates production of an active glycosylated rhPDGF-AA in large amounts for further research and drug development."
For more information on this research see: High level expression, efficient purification and bioactivity assay of recombinant human platelet-derived growth factor AA dimer (PDGF-AA) from methylotrophic yeast Pichia pastoris. Protein Expression and Purification, 2013;91(2):221-227. Protein Expression and Purification can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Protein Expression and Purification - www.elsevier.com/wps/product/cws_home/622935)
The news correspondents report that additional information may be obtained from H.B. Li, University of Florida, Dept. of Mol Genet & Microbiol, Gainesville, FL 32611, United States. Additional authors for this research include X.Y. Hui, S. Yang, X. Hu, X.F. Tang, P. Li, S.W. Li, L.J. Yang, S.G. Jin, Y. Wang, A.M. Xu and D.H. Wu (see also Intercellular Signaling Peptides and Proteins).
Keywords for this news article include: Florida, Gainesville, United States, Blood Proteins, North and Central America, Platelet-Derived Growth Factor, Intercellular Signaling Peptides and Proteins
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