By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Gram-Positive Bacteria have been published. According to news reporting originating from Fukuoka, Japan, by NewsRx correspondents, research stated, "Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3."
Our news editors obtained a quote from the research from Kyushu University, "The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins."
According to the news editors, the research concluded: "The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions."
For more information on this research see: Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system. Journal of Bioscience and Bioengineering, 2012;114(5):490-6. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
The news editors report that additional information may be obtained by contacting R.H. Perez, Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Dept. of Bioscience and Biotechnology, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Additional authors for this research include K. Himeno, N. Ishibashi, Y. Masuda, T. Zendo, K. Fujita, P. Wilaipun, V. Leelawatcharamas, J. Nakayama and K. Sonomoto (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Japan, Fukuoka, Bacteriocins, Enterococcaceae, Lactobacillales, Bacterial Proteins, Enterococcus faecium, Gram Positive Bacteria, Gram-Positive Bacteria, Pore Forming Cytotoxic Proteins.
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