By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Capillary Electrophoresis are discussed in a new report. According to news reporting originating from Taejon, South Korea, by NewsRx correspondents, research stated, "We describe a capillary electrophoresis-mass spectrometry (CE-MS) method for newborn screening of a representative amino acid metabolic disease, namely, phenylketonuria (PKU). Underivatized phenylalanine and tyrosine in a dried blood spot (DBS) were simultaneously determined by CE-MS equipped with an ionophore membrane-packed sheathless electrospray ionization interface, which was developed by our group."
Our news editors obtained a quote from the research from the Korea Research Institute of Standards and Science, "The method was optimized for rapid determination of the underivatized amino acids, phenylalanine and tyrosine extracted from a DBS. Under the optimized conditions, the limit of detection of phenylalanine and tyrosine (signal-to-noise ratio, 3) was 0.03 and 0.07 mg/L in DBS, respectively, with a CE run time of less than 3 min. For repeated runs of a sample, coefficients of variation (CVs) for migration time were less than 3.7 %, whereas CVs for the area ratio under the curve were 2.1 and 2.9 % for 20 consecutive runs of 49.5 mg/kg Phe and 36.2 mg/kg Tyr, respectively. However, the relative standard deviations of intra- and interday assays for DBS samples were < 6.2 and < 5.8 %, respectively, which were substantially due to sample extraction from DBS. The analytical method was applied to real clinical samples of Korean neonates, and results were compared with those of conventional methods for PKU diagnosis, which required reference analytical methods such as isotope dilution CE-MS or high-performance liquid chromatography-mass spectrometry for quality assurance of the conventional kit-based assays."
According to the news editors, the research concluded: "The distinct advantages of high sensitivity and extremely low sample volume, as well as a simple, easy, and economic sample pretreatment, were demonstrated for the proposed method."
For more information on this research see: Amino acid analysis of dried blood spots for diagnosis of phenylketonuria using capillary electrophoresis-mass spectrometry equipped with a sheathless electrospray ionization interface. Analytical and Bioanalytical Chemistry, 2013;405(25):8063-8072. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
The news editors report that additional information may be obtained by contacting J.S. Jeong, Korea Res Inst Stand & Sci, Div Metrol Qual Life, Center Bioanal, Taejon 305340, South Korea. Additional authors for this research include S.K. Kim and S.R. Park (see also Capillary Electrophoresis).
Keywords for this news article include: Asia, Taejon, Peptides, Proteins, Tyrosine, South Korea, Phenylalanine, Aromatic Amino Acids, Essential Amino Acids, Capillary Electrophoresis
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